Signatures in the gut microbiota of Japanese infants who developed food allergies in early childhood

Study information

Needs review

study design

prospective cohort

Citation

PMID PubMed identifier for scientific articles.

28903469

DOI Digital object identifier for electronic documents.

10.1093/femsec/fix099

URI

Authors

Tanaka M, Korenori Y, Washio M, Kobayashi T, Momoda R, Kiyohara C, Kuroda A, Saito Y, Sonomoto K, Nakayama J

Journal

FEMS microbiology ecology

Year

2017

Keywords:

16S rRNA gene, Clostridium, fecal microbiota, food allergies, infant

Bacterial colonization in infancy is considered crucial for the development of the immune system. Recently, there has been a drastic increase in childhood allergies in Japan. Therefore, we conducted a prospective study with 56 infants on the relationship between gut microbiota in the first year of life and the development of allergies during the first 3 years. In the lactation period, organic acid producers such as Leuconostoc, Weissella and Veillonella tended to be underrepresented in subjects who developed food allergies (FA, n = 14) within the first two years. In the weaning period, children in the FA group were highly colonized by unclassified Enterobacteriaceae and two Clostridium species closely related to Clostridium paraputrificum and C. tertium, and the whole tree phylogenetic diversity index was significantly lower in the FA group. All of these differences in the weaning period were statistically significant, even after adjusting for potential confounding factors. A higher abundance of unclassified Enterobacteriaceae was also found in the other allergic group (n = 15), whereas the two Clostridium species were highly specific to the FA group. The mode of action of these Clostridium species in childhood food allergies remains unknown, warranting further investigation.

Experiment 1

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Curated date: 2021/01/10

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Subjects

Location of subjects: Japan

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Food allergy Food Hypersensitivity,Food intolerance,Food allergy,food allergy

Group 0 name Corresponds to the control (unexposed) group for case-control studies: non-allergy

Group 1 name Corresponds to the case (exposed) group for case-control studies: infant with food allergy

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: infant with food allergy

Group 0 sample size Number of subjects in the control (unexposed) group: 27

Group 1 sample size Number of subjects in the case (exposed) group: 14

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V1-V2

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Richness Number of species: unchanged

Signature 1

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Curator: Lucy Mellor

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Source: Figure 2, text

Description: Relative abundance of gut microbiota of infants at 2 months of age in food allergy (FA) and non-allergy (NA) groups

Abundance in Group 1: decreased abundance in infant with food allergy

NCBI	Quality Control	Links
Leuconostoc
Weissella
Veillonella

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Signature 2

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Source: Figure 4c, text

Description: Relative abundances of genera in infants at 1 year of age which were statistically signficant differences between the food allergy (FA) and non-allergy (NA) groups

Abundance in Group 1: increased abundance in infant with food allergy

NCBI	Quality Control	Links
Enterobacteriaceae
Clostridium

Revision editor(s): WikiWorks