Fecal microbiome signatures are different in food-allergic children compared to siblings and healthy children
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Kourosh A, Luna RA, Balderas M, Nance C, Anagnostou A, Devaraj S, Davis CM
Journal
Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology
Year
2018
Keywords:
T regulatory cells, environment, food allergy, genetics, microbiome
BACKGROUND: Intestinal microbes have been shown to influence predisposition to atopic disease, including food allergy. The intestinal microbiome of food-allergic children may differ in significant ways from genetically similar non-allergic children and age-matched controls. The aim was to characterize fecal microbiomes to identify taxa that may influence the expression of food allergy. METHODS: Stool samples were collected from children with IgE-mediated food allergies, siblings without food allergy, and non-allergic controls. Stool microbiome characterization was performed via next-generation sequencing (Illumina) of the V1V3 and V4 variable regions of the 16S rRNA gene. Bacterial diversity, evenness, richness, and relative abundance of the operational taxonomic units (OTUs) were evaluated using QIIME. ANOVA and Welch's t test were utilized to compare groups. RESULTS: Sixty-eight children were included: food-allergic (n = 22), non-food-allergic siblings (n = 25), and controls (n = 21). When comparing fecal microbial communities across groups, differences were noted in Rikenellaceae (P = .035), Actinomycetaceae (P = .043), and Pasteurellaceae (P = .018), and nine other distinct OTUs. Food-allergic subjects had enrichment for specific microbes within the Clostridia class and Firmicutes phylum (Oscillobacter valericigenes, Lachnoclostridium bolteae, Faecalibacterium sp.) compared to siblings and controls. Identification of Clostridium sp. OTUs revealed differences in specific Clostridia drive the separation of the allergic from the siblings and controls. Alistipes sp. were enriched in non-allergic siblings. CONCLUSIONS: Comparisons in the fecal microbiome of food-allergic children, siblings, and healthy children point to key differences in microbiome signatures, suggesting the role of both genetic and environmental contributors in the manifestation of food-allergic disease.
Experiment 1
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled (if applicable)
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- food allergy Food Hypersensitivity,Food intolerance,food allergy
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy control
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- child with food allergy
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- child with food allergy
- Group 0 sample size Number of subjects in the control (unexposed) group
- 21
- Group 1 sample size Number of subjects in the case (exposed) group
- 22
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- Matched on Factors on which subjects have been matched on in a case-control study
- age, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Source: Figure 3, Figure 4, Figure 5
Description: Differentially abundant operational taxonomic units (OTUs) obsereved between food-allergic children and healthy controls
Abundance in Group 1: increased abundance in child with food allergy
| NCBI | Quality Control | Links |
|---|---|---|
| Faecalibacterium sp. | ||
| Oscillibacter valericigenes |
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Source: Figure 3, Figure 4, Figure 5
Description: Differentially abundant operational taxonomic units (OTUs) obsereved between food-allergic children and healthy controls
Abundance in Group 1: decreased abundance in child with food allergy
| NCBI | Quality Control | Links |
|---|---|---|
| Blautia sp. | ||
| Haemophilus parainfluenzae |
Experiment 2
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy sibling
- Group 0 sample size Number of subjects in the control (unexposed) group
- 25
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Matched on Factors on which subjects have been matched on in a case-control study
- Not specified
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Source: Figure 3, Figure 4, Figure 5
Description: Differentially abundant operational taxonomic units (OTUs) obsereved between food-allergic children and their siblings
Abundance in Group 1: increased abundance in child with food allergy
| NCBI | Quality Control | Links |
|---|---|---|
| Oscillibacter valericigenes | ||
| Faecalibacterium sp. |
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Source: Figure 3, Figure 4, Figure 5
Description: Differentially abundant operational taxonomic units (OTUs) obsereved between food-allergic children and their siblings
Abundance in Group 1: decreased abundance in child with food allergy
| NCBI | Quality Control | Links |
|---|---|---|
| Haemophilus parainfluenzae | ||
| Blautia sp. | ||
| Alistipes putredinis | ||
| Alistipes sp. |
Experiment 3
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy control
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- healthy sibling
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- sibling with no food allergy
- Group 0 sample size Number of subjects in the control (unexposed) group
- 21
- Group 1 sample size Number of subjects in the case (exposed) group
- 25
Lab analysis
Statistical Analysis
- Matched on Factors on which subjects have been matched on in a case-control study
- age, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Source: Figure 3, Figure 4, Figure 5
Description: Differentially abundant operational taxonomic units (OTUs) obsereved between non-food-allergic siblings and healthy controls
Abundance in Group 1: increased abundance in healthy sibling
| NCBI | Quality Control | Links |
|---|---|---|
| Oscillibacter valericigenes | ||
| Faecalibacterium sp. | ||
| Alistipes putredinis | ||
| Alistipes sp. |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Source: Figure 3, Figure 4, Figure 5
Description: Differentially abundant operational taxonomic units (OTUs) obsereved between non-food-allergic siblings and healthy controls
Abundance in Group 1: decreased abundance in healthy sibling
| NCBI | Quality Control | Links |
|---|---|---|
| Blautia sp. | ||
| Haemophilus parainfluenzae |
Experiment 4
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- age age
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- <7 years
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- 7-18 years
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- child age 7-18
- Group 0 sample size Number of subjects in the control (unexposed) group
- 26
- Group 1 sample size Number of subjects in the case (exposed) group
- 42
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- Not specified
- Matched on Factors on which subjects have been matched on in a case-control study
- Not specified
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Source: Text
Description: Differentially abundant taxons in children <7 years compared to children 7-18 years
Abundance in Group 1: decreased abundance in 7-18 years
| NCBI | Quality Control | Links |
|---|---|---|
| Bacillota | ||
| Pseudomonadota | ||
| Erysipelotrichaceae | ||
| Lachnospiraceae | ||
| Peptostreptococcaceae |
Revision editor(s): WikiWorks
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