A Walnut-Enriched Diet Affects Gut Microbiome in Healthy Caucasian Subjects: A Randomized, Controlled Trial/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by Atrayees on 2023-7-18
Subjects
- Location of subjects
- Germany
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Diet Dietary,Diets,Diet,diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- replace both
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- carbohydrate replacement (with walnuts)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- before walnut consumption
- Group 0 sample size Number of subjects in the control (unexposed) group
- 135
- Group 1 sample size Number of subjects in the case (exposed) group
- 135
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- excluded those on "antibiotic therapy"
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Kruskall-Wallis
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, body mass index, smoking behavior, triglycerides, Confounders controlled for: "LDL cholesterol" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.LDL cholesterol
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-7-18
Source: 5a and text
Description: Most abundant Operational Taxonomic Units for both walnut and control phase at genus level. Significant different OTUs are marked with by using * and p-values. pvalues were calculated using a pairwise Fisher test.
Abundance in Group 1: decreased abundance in carbohydrate replacement (with walnuts)
| NCBI | Quality Control | Links |
|---|---|---|
| Gemmiger | ||
| Fusicatenibacter | ||
| Bacteroides |
