Microbial Community of Healthy Thai Vegetarians and Non-Vegetarians, Their Core Gut Microbiota, and Pathogen Risk

Study information

Reviewed Marked as Reviewed by Peace Sandy on 2024-2-5

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

27381339

DOI Digital object identifier for electronic documents.

10.4014/jmb.1603.03057

URI

Authors

Ruengsomwong S, La-Ongkham O, Jiang J, Wannissorn B, Nakayama J, Nitisinprasert S

Journal

Journal of microbiology and biotechnology

Year

2016

Keywords:

Enterobacteriaceae, Fecal microbiota, Prevotella, core gut microbiota, pathogen risk, pyrosequencing

Pyrosequencing analysis of intestinal microflora from healthy Thai vegetarians and non-vegetarians exhibited 893 OTUs covering 189 species. The strong species indicators of vegetarians and non-vegetarians were Prevotella copri and Bacteroides vulgatus as well as bacteria close to Escherichia hermanii with % relative abundance of 16.9 and 4.5-4.7, respectively. Core gut microbiota of the vegetarian and non-vegetarian groups consisted of 11 and 20 different bacterial species, respectively, belonging to Actinobacteria, Firmicutes, and Proteobacteria commonly found in both groups. Two species, Faecalibacterium prausnitzii and Gemmiger formicilis, had a prevalence of 100% in both groups. Three species, Clostridium nexile, Eubacterium eligens, and P. copri, showed up in most vegetarians, whereas more diversity of Collinsella aerofaciens, Ruminococcus torques, various species of Bacteroides, Parabacteroides, Escherichia, and different species of Clostridium and Eubacterium were found in most non-vegetarians. Considering the correlation of personal characters, consumption behavior, and microbial groups, the age of non-vegetarians showed a strong positive correlation coefficient of 0.54 (p = 0.001) to Bacteroides uniformis but exhibited a moderate one to Alistipes finegoldii and B. vulgatus. Only a positive moderate correlation of body mass index and Parabacteroides distasonis appeared. Based on the significant abundance of potential pathogens, the microbiota of the non-vegetarian group showed an abundance of potential pathogen varieties of Bilophila wadsworthia, Escherichia coli, and E. hermannii, whereas that of the vegetarian group served for only Klebsiella pneumoniae. These results implied that the microbiota of vegetarians with high abundance of P. copri and low potential pathogen variety would be a way to maintain good health in Thais.

Experiment 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-5

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Peace Sandy

Subjects

Location of subjects: Thailand

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Diet Dietary,Diets,Diet,diet

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy non-vegetarian

Group 1 name Corresponds to the case (exposed) group for case-control studies: Healthy vegetarian

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: The vegetarian volunteers consisted of ovo-lacto vegetarians, lacto-vegetarians, an ovo-vegetarian, and vegans. They had all been vegetarians for at least 3 years before participating in this study.

Group 0 sample size Number of subjects in the control (unexposed) group: 36

Group 1 sample size Number of subjects in the case (exposed) group: 36

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 1 month

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V6-V8

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Roche454

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-5

Curated date: 2021/01/10

Curator: Valentina Pineda

Revision editor(s): WikiWorks, Peace Sandy

Source: Fig. 3 , Table1 and Table 3

Description: Abundance of gut microbiota at the family, genus and species levels detected in both vegetarian and non-vegetarian groups.

Abundance in Group 1: increased abundance in Healthy vegetarian

NCBI	Quality Control	Links
Streptococcus
Prevotella
Klebsiella
Faecalibacterium
Roseburia
Lachnospiraceae
Blautia
Klebsiella pneumoniae
Prevotella corporis

Revision editor(s): WikiWorks, Peace Sandy

Signature 2

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-5

Curated date: 2021/01/10

Curator: Valentina Pineda

Revision editor(s): WikiWorks, Peace Sandy

Source: Fig. 3 , Table3 and Table 1

Description: Abundance of gut microbiota at the family, genus and species levels detected in both vegetarian and non-vegetarian groups.

Abundance in Group 1: decreased abundance in Healthy vegetarian

NCBI	Quality Control	Links
Akkermansia
Alistipes
Bacteroides
Bifidobacterium
Clostridium
Coprococcus
Dialister
Megamonas
Oscillibacter
Parabacteroides
Phascolarctobacterium
Rikenellaceae
Salmonella

Revision editor(s): WikiWorks, Peace Sandy