Linking cervicovaginal immune signatures, HPV and microbiota composition in cervical carcinogenesis in non-Hispanic and Hispanic women

From BugSigDB
Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Łaniewski P, Barnes D, Goulder A, Cui H, Roe DJ, Chase DM, Herbst-Kralovetz MM
Journal
Scientific reports
Year
2018
While high-risk human papillomavirus (HPV) infection is a well-established risk factor for cervical cancer, there are likely other factors within the local microenvironment that contribute to cervical carcinogenesis. Here we investigated relationships between HPV, vaginal pH, vaginal microbiota (VMB) composition, level of genital immune mediators and severity of cervical neoplasm. We enrolled women with low- and high-grade cervical dysplasia (LGD, HGD), invasive cervical carcinoma (ICC), and healthy controls. HPV16, HPV45, HPV58, and HPV31 were the most prevalent in our cohort with HPV16 and HPV31 genotypes more prevalent in Hispanics. Vaginal pH was associated with ethnicity and severity of cervical neoplasm. Lactobacillus dominance decreased with the severity of cervical neoplasm, which correlated with elevated vaginal pH. Hispanic ethnicity was also associated with decreased Lactobacillus dominance. Furthermore, Sneathia was enriched in all precancerous groups, ICC, abnormal pH and Hispanic origin. Patients with ICC, but not LGD and HGD, exhibited increased genital inflammatory scores and elevated specific immune mediators. Notably, IL-36γ was significantly associated with ICC. Our study revealed local, host immune and microbial signatures associated with cervical carcinogenesis and provides an initial step to understanding the complex interplay between mucosal inflammation, HPV persistence and the VMB.

Experiment 1


Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Vagina Vagina,vagina,Distal oviductal region,Distal portion of oviduct,Vaginae
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Human papilloma virus infection HPV,Human Papilloma Virus Infection,Human papilloma Virus infection,human papilloma virus infection,Human papillomavirus caused disease or disorder,Human papillomavirus disease or disorder,Human Papillomavirus Infection,Human Papillomavirus infection,Human papillomavirus infectious disease,Human papilloma virus infection
Group 0 name Corresponds to the control (unexposed) group for case-control studies
HPV-
Group 1 name Corresponds to the case (exposed) group for case-control studies
HPV+
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
HPV+ determined by the Linear Array HPV Genotyping Tests
Group 0 sample size Number of subjects in the control (unexposed) group
20
Group 1 sample size Number of subjects in the case (exposed) group
31
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes


Signature 1

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the HPV+ group when compared to HPV-negative control prior to adjusting

Abundance in Group 1: increased abundance in HPV+

NCBI Quality ControlLinks
Prevotella
Sneathia sanguinegens

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the HPV+ group when compared to HPV-negative control prior to adjusting

Abundance in Group 1: decreased abundance in HPV+

NCBI Quality ControlLinks
Actinomyces
Anaerococcus lactolyticus
Bifidobacterium
Dialister
Fenollaria massiliensis
Fusobacterium nucleatum
Gardnerella
Lactobacillus
Lactobacillus gasseri
Mobiluncus mulieris
Peptoniphilus grossensis
Streptococcus anginosus

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 2


Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Cervical glandular intraepithelial neoplasia Cervical glandular intraepithelial neoplasia,cervical glandular intraepithelial neoplasia
Group 1 name Corresponds to the case (exposed) group for case-control studies
low grade dysplasia
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
low grade dysplasia confirmed by histology of colonoscopy-directed biopsy
Group 1 sample size Number of subjects in the case (exposed) group
12

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the low grade dysplasia group when compared to HPV-negative control prior to adjusting

Abundance in Group 1: increased abundance in low grade dysplasia

NCBI Quality ControlLinks
Fannyhessea vaginae
Gemella asaccharolytica
Mobiluncus mulieris
Parvimonas
Sneathia sanguinegens

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the low grade dysplasia group when compared to HPV-negative control prior to adjusting

Abundance in Group 1: decreased abundance in low grade dysplasia

NCBI Quality ControlLinks
Anaerococcus obesiensis
Brenneria
Campylobacter ureolyticus
Escherichia
Finegoldia magna
Fusobacterium nucleatum
Lactobacillus
Lactobacillus crispatus
Lactobacillus gasseri
Peptoniphilus
Peptoniphilus coxii
Peptoniphilus duerdenii
Peptoniphilus grossensis
Shigella
Streptococcus
Streptococcus anginosus

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 3


Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
high grade dysplasia
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
high grade dysplasia confirmed by histology of colonoscopy-directed biopsy
Group 1 sample size Number of subjects in the case (exposed) group
27

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the high grade dysplasia group when compared to HPV-negative control prior to adjusting

Abundance in Group 1: increased abundance in high grade dysplasia

NCBI Quality ControlLinks
Atopobium
Clostridium
Fannyhessea vaginae
Gardnerella
Hoylesella buccalis
Megasphaera
Parvimonas
Prevotella amnii
Shuttleworthella
Sneathia
Sneathia sanguinegens
Streptococcus agalactiae

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the high grade dysplasia group when compared to HPV-negative control prior to adjusting

Abundance in Group 1: decreased abundance in high grade dysplasia

NCBI Quality ControlLinks
Anaerococcus lactolyticus
Anaerococcus obesiensis
Bifidobacterium
Dialister
Dialister propionicifaciens
Fenollaria massiliensis
Finegoldia magna
Lactobacillus
Lactobacillus gasseri
Peptoniphilus coxii
Peptoniphilus duerdenii
Peptoniphilus grossensis
Peptoniphilus lacrimalis
Porphyromonas
Prevotella bivia
Prevotella corporis
Prevotella disiens
Streptococcus
Ureaplasma

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 4


Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Cervical cancer cancer of uterine cervix,cervical neoplasm,cervix cancer,cervix uteri cancer,malignant cervical neoplasm,malignant cervical tumor,malignant cervix neoplasm,malignant cervix tumor,malignant cervix uteri neoplasm,malignant cervix uteri tumor,malignant neoplasm of cervix,malignant neoplasm of cervix uteri,malignant neoplasm of the cervix,malignant neoplasm of the cervix uteri,malignant neoplasm of the uterine cervix,malignant neoplasm of uterine cervix,malignant tumor of cervix,malignant tumor of cervix uteri,malignant tumor of the cervix,malignant tumor of the cervix uteri,malignant tumor of the uterine cervix,malignant tumor of uterine cervix,malignant uterine cervix neoplasm,malignant uterine cervix tumor,tumor of the cervix uteri,uterine cervical neoplasm,uterine cervix cancer,Cervical cancer,cervical cancer
Group 1 name Corresponds to the case (exposed) group for case-control studies
invasive cervical carcinoma
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
invasive cervical carcinoma confirmed by histology of colonoscopy-directed biopsy
Group 1 sample size Number of subjects in the case (exposed) group
10

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the invasive cervical carcinoma group when compared to HPV-negative control prior to adjusting

Abundance in Group 1: increased abundance in invasive cervical carcinoma

NCBI Quality ControlLinks
Sneathia sanguinegens

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the invasive cervical carcinoma group when compared to HPV-negative control prior to adjusting

Abundance in Group 1: decreased abundance in invasive cervical carcinoma

NCBI Quality ControlLinks
Anaerococcus lactolyticus
Bifidobacterium
Brenneria
Corynebacterium
Dialister
Dialister propionicifaciens
Escherichia
Fenollaria massiliensis
Fusobacterium nucleatum
Howardella
Lactobacillus
Lactobacillus crispatus
Lactobacillus gasseri
Peptoniphilus coxii
Peptoniphilus duerdenii
Peptoniphilus grossensis
Prevotella bivia
Prevotella corporis
Shigella
Streptococcus
Streptococcus anginosus
Ureaplasma
Winkia neuii subsp. anitrata

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 5


Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Human papilloma virus infection HPV,Human Papilloma Virus Infection,Human papilloma Virus infection,human papilloma virus infection,Human papillomavirus caused disease or disorder,Human papillomavirus disease or disorder,Human Papillomavirus Infection,Human Papillomavirus infection,Human papillomavirus infectious disease,Human papilloma virus infection
Group 1 name Corresponds to the case (exposed) group for case-control studies
HPV+
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
HPV+ determined by the Linear Array HPV Genotyping Tests
Group 1 sample size Number of subjects in the case (exposed) group
31

Lab analysis

Statistical Analysis

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
age, body mass index, ethnic group


Signature 1

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the HPV+ group when compared to HPV-negative control after adjusting for age, BMI and ethnicity

Abundance in Group 1: increased abundance in HPV+

NCBI Quality ControlLinks
Lactobacillus
Megasphaera
Olsenella
Peptostreptococcus anaerobius
Prevotella
Shuttleworthella

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the HPV+ group when compared to HPV-negative control after adjusting for age, BMI and ethnicity

Abundance in Group 1: decreased abundance in HPV+

NCBI Quality ControlLinks
Aerococcus
Anaerococcus
Anaerococcus obesiensis
Bifidobacterium
Gardnerella
Hoylesella timonensis
Peptoniphilus grossensis
Prevotella bivia

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 6


Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Cervical glandular intraepithelial neoplasia Cervical glandular intraepithelial neoplasia,cervical glandular intraepithelial neoplasia
Group 1 name Corresponds to the case (exposed) group for case-control studies
low grade dysplasia
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
low grade dysplasia confirmed by histology of colonoscopy-directed biopsy
Group 1 sample size Number of subjects in the case (exposed) group
12

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the low grade dysplasia group when compared to HPV-negative control after adjusting for age, BMI and ethnicity

Abundance in Group 1: increased abundance in low grade dysplasia

NCBI Quality ControlLinks
Anaerococcus lactolyticus
Bifidobacterium
Clostridium
Dialister
Gemella asaccharolytica
Hoylesella buccalis
Lactobacillus
Lactobacillus gasseri
Lactobacillus iners
Mobiluncus mulieris
Peptostreptococcus anaerobius
Porphyromonas
Prevotella
Prevotella amnii
Prevotella disiens
Sneathia
Sneathia sanguinegens
Winkia neuii subsp. anitrata

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the low grade dysplasia group when compared to HPV-negative control after adjusting for age, BMI and ethnicity

Abundance in Group 1: decreased abundance in low grade dysplasia

NCBI Quality ControlLinks
Lactobacillus
Lactobacillus crispatus
Peptoniphilus coxii

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 7


Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
high grade dysplasia
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
high grade dysplasia confirmed by histology of colonoscopy-directed biopsy
Group 1 sample size Number of subjects in the case (exposed) group
27

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the high grade dysplasia group when compared to HPV-negative control after adjusting for age, BMI and ethnicity

Abundance in Group 1: increased abundance in high grade dysplasia

NCBI Quality ControlLinks
Clostridium
Gardnerella
Lactobacillus iners
Megasphaera
Parvimonas
Prevotella amnii
Shuttleworthella
Sneathia
Sneathia sanguinegens

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): Fatima, WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the high grade dysplasia group when compared to HPV-negative control after adjusting for age, BMI and ethnicity

Abundance in Group 1: decreased abundance in high grade dysplasia

NCBI Quality ControlLinks
Actinotignum schaalii
Aerococcus
Anaerococcus
Anaerococcus lactolyticus
Anaerococcus obesiensis
Bifidobacterium
Campylobacter ureolyticus
Corynebacterium coyleae
Dialister
Ezakiella coagulans
Fenollaria massiliensis
Gallicola
Gardnerella
Howardella
Hoylesella buccalis
Hoylesella timonensis
Peptoniphilus coxii
Peptoniphilus duerdenii
Peptoniphilus grossensis
Peptoniphilus lacrimalis
Porphyromonas
Prevotella bivia

Revision editor(s): Fatima, WikiWorks, ChiomaBlessing

Experiment 8


Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Cervical cancer cancer of uterine cervix,cervical neoplasm,cervix cancer,cervix uteri cancer,malignant cervical neoplasm,malignant cervical tumor,malignant cervix neoplasm,malignant cervix tumor,malignant cervix uteri neoplasm,malignant cervix uteri tumor,malignant neoplasm of cervix,malignant neoplasm of cervix uteri,malignant neoplasm of the cervix,malignant neoplasm of the cervix uteri,malignant neoplasm of the uterine cervix,malignant neoplasm of uterine cervix,malignant tumor of cervix,malignant tumor of cervix uteri,malignant tumor of the cervix,malignant tumor of the cervix uteri,malignant tumor of the uterine cervix,malignant tumor of uterine cervix,malignant uterine cervix neoplasm,malignant uterine cervix tumor,tumor of the cervix uteri,uterine cervical neoplasm,uterine cervix cancer,Cervical cancer,cervical cancer
Group 1 name Corresponds to the case (exposed) group for case-control studies
invasive cervical carcinoma
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
invasive cervical carcinoma confirmed by histology of colonoscopy-directed biopsy
Group 1 sample size Number of subjects in the case (exposed) group
10

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Fatima on 2021/10/14

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): Lwaldron, Cynthia Anderson, WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in the invasive cervical carcinoma group when compared to HPV-negative control after adjusting for age, BMI, and ethnicity

Abundance in Group 1: decreased abundance in invasive cervical carcinoma

NCBI Quality ControlLinks
Anaerococcus
Bifidobacterium
Gardnerella
Lactobacillus
Lactobacillus crispatus
Moryella
Peptoniphilus grossensis
Prevotella bivia

Revision editor(s): Lwaldron, Cynthia Anderson, WikiWorks, ChiomaBlessing

Experiment 9


Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Human papilloma virus infection , Cervical cancer HPV,Human Papilloma Virus Infection,Human papilloma Virus infection,human papilloma virus infection,Human papillomavirus caused disease or disorder,Human papillomavirus disease or disorder,Human Papillomavirus Infection,Human Papillomavirus infection,Human papillomavirus infectious disease,Human papilloma virus infection,cancer of uterine cervix,cervical neoplasm,cervix cancer,cervix uteri cancer,malignant cervical neoplasm,malignant cervical tumor,malignant cervix neoplasm,malignant cervix tumor,malignant cervix uteri neoplasm,malignant cervix uteri tumor,malignant neoplasm of cervix,malignant neoplasm of cervix uteri,malignant neoplasm of the cervix,malignant neoplasm of the cervix uteri,malignant neoplasm of the uterine cervix,malignant neoplasm of uterine cervix,malignant tumor of cervix,malignant tumor of cervix uteri,malignant tumor of the cervix,malignant tumor of the cervix uteri,malignant tumor of the uterine cervix,malignant tumor of uterine cervix,malignant uterine cervix neoplasm,malignant uterine cervix tumor,tumor of the cervix uteri,uterine cervical neoplasm,uterine cervix cancer,Cervical cancer,cervical cancer
Group 0 name Corresponds to the control (unexposed) group for case-control studies
abnormal pH
Group 1 name Corresponds to the case (exposed) group for case-control studies
normal pH
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
HPV+ determined by the Linear Array HPV Genotyping Tests, low grade dysplasia,high grade dysplasia, invasive cervical carcinoma confirmed by histology of colonoscopy-directed biopsy,
Group 1 sample size Number of subjects in the case (exposed) group
74

Lab analysis

Statistical Analysis

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
Not specified


Signature 1

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in patients with abnormal pH vs. normal pH

Abundance in Group 1: increased abundance in normal pH

NCBI Quality ControlLinks
Atopobium
Bifidobacterium
Clostridium
Enterococcus
Fusobacterium nucleatum
Gardnerella
Gemella
Megasphaera
Metamycoplasma hominis
Mobiluncus mulieris
Moryella
Parvimonas
Peptostreptococcus
Prevotella
Prevotella amnii
Prevotella disiens
Shuttleworthella
Sneathia
Sneathia sanguinegens
Staphylococcus
Streptococcus
Streptococcus agalactiae
Veillonella
Veillonella montpellierensis

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in patients with abnormal pH vs. normal pH

Abundance in Group 1: decreased abundance in normal pH

NCBI Quality ControlLinks
Brenneria
Clostridium
Escherichia
Lactobacillus
Lactobacillus gasseri
Shigella

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 10


Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Human papilloma virus infection HPV,Human Papilloma Virus Infection,Human papilloma Virus infection,human papilloma virus infection,Human papillomavirus caused disease or disorder,Human papillomavirus disease or disorder,Human Papillomavirus Infection,Human Papillomavirus infection,Human papillomavirus infectious disease,Human papilloma virus infection
Group 0 name Corresponds to the control (unexposed) group for case-control studies
non-Hispanic
Group 1 name Corresponds to the case (exposed) group for case-control studies
Hispanic
Group 0 sample size Number of subjects in the control (unexposed) group
53
Group 1 sample size Number of subjects in the case (exposed) group
47

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in Hispanic vs. non-Hispanic group

Abundance in Group 1: increased abundance in Hispanic

NCBI Quality ControlLinks
Aerococcus
Atopobium
Enterococcus
Fannyhessea vaginae
Gardnerella
Megasphaera
Mobiluncus mulieris
Moryella
Porphyromonas
Shuttleworthella
Sneathia
Sneathia sanguinegens
Streptococcus
Streptococcus anginosus
Veillonella
Veillonella montpellierensis

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4

Description: Enrichment in bacterial taxa in Hispanic vs. non-Hispanic group

Abundance in Group 1: decreased abundance in Hispanic

NCBI Quality ControlLinks
Anaerococcus vaginalis
Hoylesella buccalis
Lactobacillus
Lactobacillus gasseri

Revision editor(s): WikiWorks, ChiomaBlessing