Microbial changes in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients/Experiment 4

EditHistoryDelete

Flag for review

Your review change was submittedDuplicate this ExperimentAdd a new Signature

Reviewed Marked as Reviewed by Atrayees on 2023-7-24

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects

Netherlands

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Mouth Adult mouth,Cavital oralis,Cavitas oris,Cavum oris,Mouth cavity,Oral region,Oral vestibule,Regio oralis,Rima oris,Stoma,Stomatodaeum,Trophic apparatus,Vestibule of mouth,Vestibulum oris,Mouth,mouth

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Oral mucositis inflammation of the mouth,oral cavity mucosal damage,oral mucosa irritation,oral mucosal damage,oral mucosal injury,oral mucosal lesions,stomatitis,Oral mucositis,oral mucositis

Group 0 name Corresponds to the control (unexposed) group for case-control studies

low or no fungi load

Group 1 name Corresponds to the case (exposed) group for case-control studies

0.1% or higher fungi load

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

patient developed uclerative oral mucositis

Group 0 sample size Number of subjects in the control (unexposed) group

31

Group 1 sample size Number of subjects in the case (exposed) group

20

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

RT-qPCR

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

No

Signature 1

EditHistoryDelete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Atrayees on 2023-7-24

Curated date: 2021/01/10

Curator: William Lam

Revision editor(s): WikiWorks, Atrayees

Source: text

Description: Principle component analysis of salivary microbial profiles based on fungal load relative to bacterial 16s rDNA

Abundance in Group 1: increased abundance in 0.1% or higher fungi load

NCBI	Quality Control	Links
Scardovia
Staphylococcus
Lactobacillus

Revision editor(s): WikiWorks, Atrayees

Signature 2

EditHistoryDelete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Atrayees on 2023-7-24

Curated date: 2021/01/10

Curator: William Lam

Revision editor(s): WikiWorks, Atrayees

Source: text

Description: Principle component analysis of salivary microbial profiles based on fungal load relative to bacterial 16s rDNA

Abundance in Group 1: decreased abundance in 0.1% or higher fungi load

NCBI	Quality Control	Links
Veillonella
Actinomyces
Prevotella
Leptotrichia
Megasphaera
Streptococcus

Revision editor(s): WikiWorks, Atrayees