Patients With LR-HPV Infection Have a Distinct Vaginal Microbiota in Comparison With Healthy Controls

Study information

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

31555603

DOI Digital object identifier for electronic documents.

10.3389/fcimb.2019.00294

URI

Authors

Zhou Y, Wang L, Pei F, Ji M, Zhang F, Sun Y, Zhao Q, Hong Y, Wang X, Tian J, Wang Y

Journal

Frontiers in cellular and infection microbiology

Year

2019

Keywords:

16S RNA sequencing, condyloma acuminatum, low-risk HPV, sexually transmitted infections, vaginal microbiome

Condyloma acuminatum (CA) is a benign epithelium hyperplasia mainly caused by human papillomavirus (HPV), which is now the second most common viral sexually transmitted infection (STI) in China. In total, 90% of CA patients are caused by the low-risk HPV 6 and 11. Aside from low-risk HPV infection there are likely other factors within the local microenvironment that contribute to CA and there has been related research before. In this study, 62 vaginal specimens were analyzed using 16S rRNA gene sequencing. The diversity of the vaginal microbiota was higher and the composition was different with LR-HPV infection. While the relative abundance of dominant Firmicutes was lower, Actinobacteria, Proteobacteria, and Fusobacteria phyla were significantly higher; at the genus level Gardnerella, Bifidobacterium, Sneathia, Hydrogenophilus, Burkholderia, and Atopobium were higher. This study firstly confirmed a more accurate and comprehensive understanding of the relationship between low-risk HPV infection and vaginal microbiota, in order to provide a theoretical basis for further research on the occurrence and development of CA.

Experiment 1

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Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Peace Sandy

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Vagina Vagina,vagina,Distal oviductal region,Distal portion of oviduct,Vaginae

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Human papilloma virus infection HPV,Human Papilloma Virus Infection,Human papilloma Virus infection,human papilloma virus infection,Human papillomavirus caused disease or disorder,Human papillomavirus disease or disorder,Human Papillomavirus Infection,Human Papillomavirus infection,Human papillomavirus infectious disease,Human papilloma virus infection

Group 0 name Corresponds to the control (unexposed) group for case-control studies: negative control

Group 1 name Corresponds to the case (exposed) group for case-control studies: low risk HPV

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: LR-HPV+ according to results of screening

Group 0 sample size Number of subjects in the control (unexposed) group: 20

Group 1 sample size Number of subjects in the case (exposed) group: 42

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Simpson Estimator of species richness and species evenness: more weight on species evenness: increased

Signature 1

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Reviewed Marked as Reviewed by Fatima on 2021/07/16

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): Lwaldron, WikiWorks, Merit

Source: Figure 4B

Description: Comparisons of vaginal bacteria between the NC and HPV-LR groups

Abundance in Group 1: increased abundance in low risk HPV

NCBI	Quality Control	Links
Actinomyces
Actinomycetaceae
Alistipes
Bifidobacterium
Bilophila
Blautia
Burkholderiaceae
Butyricimonas
Clostridia
Clostridium
Comamonadaceae
Coprobacter
Coprococcus
Dialister
Erysipelotrichaceae
Erysipelotrichales
Erysipelotrichia
Eubacteriales
Fusicatenibacter
Fusobacteriales
Fusobacteriia
Fusobacteriota
Gemmiger
Hydrogenophilaceae
Hydrogenophilales
Hydrogenophilus
Lachnospiraceae
Leptotrichiaceae
Megasphaera
Mollicutes
Mycoplasmatota
Oscillospiraceae
Pasteurella
Pasteurellaceae
Pasteurellales
Peptostreptococcaceae
Peptostreptococcus
Phascolarctobacterium
Rikenellaceae
Roseburia
Ruminococcus
Saccharofermentans
Sneathia
Sutterella
Sutterellaceae

Revision editor(s): Lwaldron, WikiWorks, Merit

Signature 2

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Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, Merit

Source: Figure 4B

Description: Comparisons of vaginal bacteria between the NC and HPV-LR groups

Abundance in Group 1: decreased abundance in low risk HPV

NCBI	Quality Control	Links
Aeromonadales
Azotobacter group
Gammaproteobacteria
Helicobacter
Helicobacteraceae
Lysobacter
Nitrososphaera
Nitrososphaeraceae
Nitrososphaerales
Nitrososphaerota
Pseudomonadales
Succinivibrionaceae
Pseudomonadaceae

Revision editor(s): WikiWorks, Merit