Gut microbiota is critical for the induction of chemotherapy-induced pain/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-22

Curated date: 2024/02/22

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Response to antibiotic Response to antibiotic,response to antibiotic
Group 0 name Corresponds to the control (unexposed) group for case-control studies
H2O mice
Group 1 name Corresponds to the case (exposed) group for case-control studies
Exposed abx mice
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice fed with antibiotic water
Group 0 sample size Number of subjects in the control (unexposed) group
10
Group 1 sample size Number of subjects in the case (exposed) group
10
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
None

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
RT-qPCR

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
ANOVA
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-22

Curated date: 2024/02/22

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Figure 1C

Description: Phylum analysis of altered microbiota in mice after antibiotic water feeding compared to mice fed with regular water

Abundance in Group 1: increased abundance in Exposed abx mice

NCBI Quality ControlLinks
Cyanobacteriota
Pseudomonadota
Bacillota

Revision editor(s): ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-22

Curated date: 2024/02/22

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing, Scholastica

Source: Figure 1C

Description: Phylum analysis of altered microbiota in mice after antibiotic water feeding compared to mice fed with regular water

Abundance in Group 1: decreased abundance in Exposed abx mice

NCBI Quality ControlLinks
Actinomycetota
Bacteroidota
Fusobacteriia
Mycoplasmatota
Verrucomicrobiae
TM7TM7

Revision editor(s): ChiomaBlessing, Scholastica