Sex-specific effects of PM2.5 maternal exposure on offspring's serum lipoproteins and gut microbiota/Experiment 6

From BugSigDB


Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colon Hindgut,Large bowel,Posterior intestine,Colon,colon
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Air pollution air pollution,Air pollution
Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 21
Group 1 name Corresponds to the case (exposed) group for case-control studies
ICR female mice exposed to PM 2.5
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
ICR mice exposed to PM 2.5
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Rukky

Source: Figure 7g & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR female mice exposed to PM 2.5

NCBI Quality ControlLinks
Deltaproteobacteria
Desulfovibrionaceae
Desulfovibrionales
Verrucomicrobiaceae
Verrucomicrobiae
Verrucomicrobiales
Verrucomicrobiota
Anaerostipes sp.

Revision editor(s): WikiWorks, Rukky

Signature 2

Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Aiyshaaaa, WikiWorks, Merit, Atrayees

Source: Figure 7g & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR female mice exposed to PM 2.5

NCBI Quality ControlLinks
Lactobacillaceae
Lactobacillus
Streptophyta

Revision editor(s): Aiyshaaaa, WikiWorks, Merit, Atrayees