Sex-specific effects of PM2.5 maternal exposure on offspring's serum lipoproteins and gut microbiota/Experiment 6

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Subjects

Location of subjects

China

Host species Species from which microbiome was sampled. Contact us to have more species added.

Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Colon Hindgut,Large bowel,Posterior intestine,Colon,colon

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Air pollution air pollution,Air pollution

Group 0 name Corresponds to the control (unexposed) group for case-control studies

mice dams exposed to filtered blanks PND 21

Group 1 name Corresponds to the case (exposed) group for case-control studies

ICR female mice exposed to PM 2.5

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

ICR mice exposed to PM 2.5

Group 0 sample size Number of subjects in the control (unexposed) group

8

Group 1 sample size Number of subjects in the case (exposed) group

8

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

No

Alpha Diversity

Chao1 Abundance-based estimator of species richness

unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness

unchanged

Richness Number of species

increased

Signature 1

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Rukky

Source: Figure 7g & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR female mice exposed to PM 2.5

NCBI	Quality Control	Links
Deltaproteobacteria
Desulfovibrionaceae
Desulfovibrionales
Verrucomicrobiaceae
Verrucomicrobiia
Verrucomicrobiales
Verrucomicrobiota
Anaerostipes sp.

Revision editor(s): WikiWorks, Rukky

Signature 2

EditHistoryDelete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Merit, Atrayees, Aiyshaaaa

Source: Figure 7g & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR female mice exposed to PM 2.5

NCBI	Quality Control	Links
Lactobacillaceae
Lactobacillus
Streptophyta

Revision editor(s): WikiWorks, Merit, Atrayees, Aiyshaaaa