Composition and metabolism of fecal microbiota from normal and overweight children are differentially affected by melibiose, raffinose and raffinose-derived fructans
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Adamberg K, Adamberg S, Ernits K, Larionova A, Voor T, Jaagura M, Visnapuu T, Alamäe T
Journal
Anaerobe
Year
2018
Keywords:
Bacteroides, Candidate prebiotic, Dietary fiber, Fructooligosaccharides, Levan, Levansucrase, Short-chain fatty acids
The aim of the study was to investigate the metabolism of non-digestible oligo- and polysaccharides by fecal microbiota, using isothermal microcalorimetry. The five tested substrates were raffinose, melibiose, a mixture of oligo- and polysaccharides produced from raffinose by levansucrase, levan synthesized from raffinose, and levan from timothy grass. Two inocula were comprised of pooled fecal samples from overweight or normal-weight children, from healthy adult volunteers and a pure culture of Bacteroides thetaiotaomicron as a reference bacterium for colon microbiota. The growth was analyzed based on the heat evolution curves, and the production of organic acids and gases. Taxonomic profiles of the microbiota were assessed by 16S rDNA sequencing. Raffinose and melibiose promoted the growth of bifidobacteria in all fecal pools. Several pool-specific substrate-related responses to raffinose and melibiose were revealed. Lactate-producing bacteria (Streptococcus and Enterococcus) became enriched in the pool of overweight children resulting in lactic acid as the major fermentation product on short saccharides. Acetic and butyric acids were prevalent at fermentation in the normal-weight pool coinciding with the enrichment of Catenibacterium. In the adult pool, the specific promotion of Bacteroides and Lachnospiraceae by levans was disclosed. In the fecal pool of normal-weight children, levans stimulated the growth of Senegalimassilia and Lachnoclostridium and this particular pool also showed the highest maximum heat production rate at levan fermentation. Levans and raffinose-derived oligosaccharides, but not raffinose and melibiose were completely fermented by a pure culture of Bacteroides thetaiotaomicron. The main conclusion from the study is that fecal microbiota of normal and overweight children have different compositions and they respond in specific manners to non-digestible oligo- and polysaccharides: raffinose, melibiose, raffinose-derived oligosaccharides and levans. The potential of the tested saccharides to support a healthy balance of colon microbiota requires further studies.
Experiment 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-30
Subjects
- Location of subjects
- Estonia
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- normal weight children
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- overweight children
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- overweight, but otherwise healthy children (7-12 years)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 9
- Group 1 sample size Number of subjects in the case (exposed) group
- 9
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-30
Curated date: 2021/01/10
Curator: Mst Afroza Parvin
Source: Figure 3, Table S2, text
Description: Composition of fecal pools used as inocula according to dominant taxa classified at the phylum or species level.
Abundance in Group 1: increased abundance in overweight children
| NCBI | Quality Control | Links |
|---|---|---|
| Agathobacter rectalis | ||
| Bifidobacterium bifidum | ||
| unclassified Coprococcus | ||
| Streptococcus equinus | ||
| Eshraghiella crossota | ||
| Peptoniphilus timonensis |
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-1-30
Curated date: 2021/01/10
Curator: Mst Afroza Parvin
Revision editor(s): WikiWorks, Folakunmi, Scholastica, Welile
Source: Figure 3, Table S2
Description: Composition of fecal pools used as inocula according to dominant taxa classified at the phylum or species level.
Abundance in Group 1: decreased abundance in overweight children
Revision editor(s): WikiWorks, Folakunmi, Scholastica, Welile
Experiment 2
Reviewed Marked as Reviewed by Folakunmi on 2024-1-30
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Adults
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Overweight children
- Group 1 sample size Number of subjects in the case (exposed) group
- 7
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-30
Source: Figure 3, Table S2
Description: Composition of fecal pools used as inocula according to dominant taxa classified at the phylum or species level.
Abundance in Group 1: increased abundance in Overweight children
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-1-30
Source: Figure 3, Table S2
Description: Composition of fecal pools used as inocula according to dominant taxa classified at the phylum or species level.
Abundance in Group 1: decreased abundance in Overweight children
| NCBI | Quality Control | Links |
|---|---|---|
| Senegalimassilia anaerobia | ||
| Coprococcus eutactus | ||
| Segatella copri | ||
| Ruminococcus callidus | ||
| Eubacterium ventriosum | ||
| unclassified Lachnospira | ||
| unclassified Christensenellaceae |
Experiment 3
Reviewed Marked as Reviewed by Folakunmi on 2024-1-30
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- normal weight children
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- healthy children with normal weight (7-12 years)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 7
- Group 1 sample size Number of subjects in the case (exposed) group
- 9
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-30
Source: Figure 3, Table S2
Description: Composition of fecal pools used as inocula according to dominant taxa classified at the species level.
Abundance in Group 1: increased abundance in normal weight children
Revision editor(s): Folakunmi, Joan Chuks
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-1-30
Source: Figure 3, Table S2
Description: Composition of fecal pools used as inocula according to dominant taxa classified at the species level.
Abundance in Group 1: decreased abundance in normal weight children
| NCBI | Quality Control | Links |
|---|---|---|
| Roseburia intestinalis | ||
| Ruminococcus callidus | ||
| unclassified Alloprevotella | ||
| uncultured Erysipelotrichaceae bacterium |
Revision editor(s): Folakunmi, Joan Chuks
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