Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection/Experiment 3

Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/13

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Smoking behavior smoking,Smoking behavior,smoking behavior

Group 0 name Corresponds to the control (unexposed) group for case-control studies: cigarette smoker

Group 1 name Corresponds to the case (exposed) group for case-control studies: E-cigarette smoker

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: participants who were 21 years or older, healthy, diagnosed with periodontal disease, and current e-cigarette users

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 1 month

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/13

Curated date: 2021/01/10

Curator: Victoria Goulbourne

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure S2

Description: Differential microbial abundance between cigarette smokers and e-cigarette smokers

Abundance in Group 1: increased abundance in E-cigarette smoker

NCBI	Quality Control	Links
Capnocytophaga gingivalis
Capnocytophaga haemolytica
Capnocytophaga sputigena
Cardiobacterium hominis
Catonella morbi
Corynebacterium durum
Eikenella corrodens
Fusobacterium periodonticum
Haemophilus haemolyticus
Haemophilus sp.
Lautropia mirabilis
Neisseria oralis
Neisseria sp.
Oscillospiraceae
Peptococcus sp.
Peptostreptococcus stomatis
Porphyromonas pasteri
Prevotella sp.
Rothia mucilaginosa
Veillonella rogosae