Oral microbiome and obesity in a large study of low-income and African-American populations

From BugSigDB
Needs review
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Yang Y, Cai Q, Zheng W, Steinwandel M, Blot WJ, Shu XO, Long J
Journal
Journal of oral microbiology
Year
2019
Keywords:
16S rRNA gene sequencing; Southern Community Cohort Study; Probiotic taxa, Oral microbiome, obesity
Few studies have evaluated the relationship of oral microbiome with obesity. We investigated the oral microbiome among 647 obese and 969 non-obese individuals from the Southern Community Cohort Study, through 16S rRNA gene sequencing in mouth rinse samples. We first investigated 16 taxa in two probiotic genera, Bifidobacterium and Lactobacillus. Among them, eight showed nominal associations with obesity (P < 0.05). Especially, Bifidobacterium (odds ratio [OR] = 0.67, 95% confidence interval [CI]:0.54, 0.83) and Bifidobacterium longum (OR = 0.57, 95% CI: 0.45, 0.73) were significantly associated with decreased obesity prevalence with false-discovery rate (FDR)-corrected P of 0.01 and 5.41 × 10-4, respectively. Multiple other bacterial taxa were also significantly associated with obesity prevalence at FDR-corrected P < 0.05. Among them, five in Firmicutes and two respectively in Actinobacteria and Proteobacteria were significantly associated with increased obesity prevalence. Significant associations with decreased obesity prevalence were observed for two taxa respectively in Actinobacteria and Firmicutes. Most of these taxa were associated with body mass index at study enrollment and weight gain during adulthood. Also, most of these associations were observed in both European- and African-Americans. Our findings indicate that multiple oral bacterial taxa, including several probiotic taxa, were significantly associated with obesity.

Experiment 1


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees, Muqtadirat, Joan Chuks

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Mouth Adult mouth,Cavital oralis,Cavitas oris,Cavum oris,Mouth cavity,Oral region,Oral vestibule,Regio oralis,Rima oris,Stoma,Stomatodaeum,Trophic apparatus,Vestibule of mouth,Vestibulum oris,Mouth,mouth
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity
Group 0 name Corresponds to the control (unexposed) group for case-control studies
obese
Group 1 name Corresponds to the case (exposed) group for case-control studies
non-obese
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
participants who were selected for four nested case-control studies to investigate the oral microbiome and those diagnosed after mouth rinse sample colection with type 2 diabetes, lung cancer, upper aero-digestive tract cancer, and colorectal cancer
Group 0 sample size Number of subjects in the control (unexposed) group
647
Group 1 sample size Number of subjects in the case (exposed) group
969
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 year

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
Logistic Regression
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
Matched on Factors on which subjects have been matched on in a case-control study
age, race, sex, smoking status
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
age, alcohol drinking, smoking behavior, race, sex, Confounders controlled for: "total energy intake" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.total energy intake, Confounders controlled for: "disease status during the follow up" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.disease status during the follow up, Confounders controlled for: "and study batch" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.and study batch, number of teeth measurement

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Victoria Goulbourne

Revision editor(s): WikiWorks

Source: Table 2

Description: Probiotic bacterial taxa showing a significantly higher prevelance in non-obese than obese individuals

Abundance in Group 1: increased abundance in non-obese

NCBI Quality ControlLinks
Bifidobacterium
Bifidobacterium longum subsp. longum
Bifidobacterium scardovii
Bifidobacterium subtile
Lactobacillus
Limosilactobacillus fermentum
Limosilactobacillus panis
Lactobacillus ultunensis

Revision editor(s): WikiWorks

Experiment 2


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Aiyshaaaa, Joan Chuks

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
non-obese
Group 1 name Corresponds to the case (exposed) group for case-control studies
obese
Group 0 sample size Number of subjects in the control (unexposed) group
969
Group 1 sample size Number of subjects in the case (exposed) group
647

Lab analysis

Statistical Analysis

Matched on Factors on which subjects have been matched on in a case-control study
age, smoking behavior, race, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Victoria Goulbourne

Revision editor(s): Atrayees, WikiWorks

Source: Table 3

Description: Common bacterial taxa showing a significantly higher prevelance in obese than in non-obese individuals

Abundance in Group 1: increased abundance in obese

NCBI Quality ControlLinks
Actinomyces sp.
Carnobacteriaceae
Gemella
Granulicatella
Granulicatella adiacens
Streptococcus cristatus

Revision editor(s): Atrayees, WikiWorks

Signature 2

Needs review

Curated date: 2021/01/10

Curator: Victoria Goulbourne

Revision editor(s): Atrayees, WikiWorks

Source: Table 4

Description: Rare taxa showing a significantly higher prevelance in obese or non-obese individuals

Abundance in Group 1: increased abundance in obese

NCBI Quality ControlLinks
Alloscardovia
Anaeroglobus
Aggregatibacter sp. oral taxon 512

Revision editor(s): Atrayees, WikiWorks