Depiction of Vaginal Microbiota in Women With High-Risk Human Papillomavirus Infection

Study information

Reviewed Marked as Reviewed by Lwaldron on 2021/08/31

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

33490017

DOI Digital object identifier for electronic documents.

10.3389/fpubh.2020.587298

URI

Authors

Wei ZT, Chen HL, Wang CF, Yang GL, Han SM, Zhang SL

Journal

Frontiers in public health

Year

2020

Keywords:

16s rRNA, bacterial vaginosis, human papillomavirus, persistent infection, vaginal microbiota

Persistent infection with the carcinogenic human papillomavirus (HPV) is a prerequisite for the progression of cervical lesions and cancer. A growing body of research has focused on the functional role of the vaginal microbiota in the persistence of HPV infection. Understanding the microbial composition and structure in women with high-risk (hr)-HPV infection may help reveal associations between the vaginal microbiota and HPV infection, and identify potential biomarkers. Our study investigated the vaginal microbial community in women with and without hr-HPV infection, by using 16s rRNA gene sequencing. We found that microbial perturbations occurred in the early phase of hr-HPV infection. Lactobacillus and Sporolactobacillus were decreased, while bacteria related to bacterial vaginosis (BV), such as Gardnerella, Prevotella, Dialister, Slackia, Actinomyces, Porphyromonas, Peptoniphilus, Anaerococcus, Peptostreptococcus, Streptococcus, Ureaplasma, Megasphaera, and Mycoplasma were increased. Our results could offer insights into the correlations between hr-HPV and the vaginal microbiota in the early infection period, and provide indications that the predominance of some BV-associated bacteria during hr-HPV infection may increase the risk for cervical neoplasia.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29

Curated date: 2021/03/19

Curator: Cynthia Anderson

Revision editor(s): Cynthia Anderson, Claregrieve1, WikiWorks, Victoria

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Vagina Vagina,vagina,Distal oviductal region,Distal portion of oviduct,Vaginae

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Human papilloma virus infection HPV,Human Papilloma Virus Infection,Human papilloma Virus infection,human papilloma virus infection,Human papillomavirus caused disease or disorder,Human papillomavirus disease or disorder,Human Papillomavirus Infection,Human Papillomavirus infection,Human papillomavirus infectious disease,Human papilloma virus infection

Group 0 name Corresponds to the control (unexposed) group for case-control studies: HPV-

Group 1 name Corresponds to the case (exposed) group for case-control studies: HPV+

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: HPV+ confirmed by Hybrid Capture II assay

Group 0 sample size Number of subjects in the control (unexposed) group: 30

Group 1 sample size Number of subjects in the case (exposed) group: 30

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 2 weeks

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Richness Number of species: unchanged

Signature 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29

Curated date: 2021/03/19

Curator: Cynthia Anderson

Revision editor(s): Cynthia Anderson

Source: Figure 2E

Description: Bacterial taxa analysis of the HPV-positive and HPV-negative groups.

Abundance in Group 1: increased abundance in HPV+

NCBI	Quality Control	Links
Mycoplasma

Revision editor(s): Cynthia Anderson

Signature 2

Edit History Delete

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29

Curated date: 2021/03/19

Curator: Cynthia Anderson

Revision editor(s): Cynthia Anderson, Claregrieve1

Source: Figure 2E

Description: Bacterial taxa analysis of the HPV-positive and HPV-negative groups.

Abundance in Group 1: decreased abundance in HPV+

NCBI	Quality Control	Links
Bacilli
Lactobacillales
Sporolactobacillaceae
Sporolactobacillus

Revision editor(s): Cynthia Anderson, Claregrieve1