Cervicovaginal microbiota dysbiosis correlates with HPV persistent infection/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Folakunmi on 2024-2-9
Curated date: 2021/03/20
Curator: Cynthia Anderson
Revision editor(s): WikiWorks, Cynthia Anderson, Peace Sandy, Folakunmi
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Uterine cervix Canalis cervicis uteri,Caudal segment of uterus,Cervical canal,Cervical canal of uterus,Cervix,Cervix of uterus,Cervix uteri,Neck of uterus,Uterine cervix,uterine cervix
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Human papilloma virus infection HPV,Human Papilloma Virus Infection,Human papilloma Virus infection,human papilloma virus infection,Human papillomavirus caused disease or disorder,Human papillomavirus disease or disorder,Human Papillomavirus Infection,Human Papillomavirus infection,Human papillomavirus infectious disease,Human papilloma virus infection
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- HPV- controls and HPV+ transient patients
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- HPV+ persistence
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- HPV infection of the same type that lasts for more than 12 months
- Group 0 sample size Number of subjects in the control (unexposed) group
- 9
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 month
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Ion Torrent
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 4
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
- Richness Number of species
- increased
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-2-6
Source: Figure 5
Description: LEfSe Analysis. A: LDA value distribution histogram
Abundance in Group 1: increased abundance in HPV+ persistence
Revision editor(s): Cynthia Anderson
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-2-6
Source: Figure 5
Description: LEfSe Analysis. A: LDA value distribution histogram
Abundance in Group 1: decreased abundance in HPV+ persistence
| NCBI | Quality Control | Links |
|---|---|---|
| Bacilli | ||
| Bacillota | ||
| Lactobacillaceae | ||
| Lactobacillales | ||
| Lactobacillus | ||
| Lactobacillus iners |
Revision editor(s): Cynthia Anderson, Folakunmi
