Characterization and Analysis of the Skin Microbiota in Rosacea: A Case-Control Study/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29
Curated date: 2021/05/29
Curator: Madhubani Dey
Revision editor(s): WikiWorks, Rimsha, Madhubani Dey, Claregrieve1
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Nose , Skin of cheek Nasal sac,Nasus,Nose,Olfactory apparatus,Peripheral olfactory organ,Proboscis,nose,Cheek skin,Skin of cheek,skin of cheek
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Rosacea acne rosacea,Acne roscea,acne roscea,Acne, erythematosa,acne, erythematosa,rosacea,Rosacea
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Participants with papulopustular rosacea (PPR)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants with rosacea included individuals with diagnosed papulopustular rosacea (PPR): severity of rosacea assessed using the National Rosacea Society clinical grading system ranged from mild to moderate
- Group 0 sample size Number of subjects in the control (unexposed) group
- 19
- Group 1 sample size Number of subjects in the case (exposed) group
- 19
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 4 weeks for topical antibiotics, corticosteroids, and other anti-inflammatory medications, within 8 weeks for systemic antibiotics, corticosteroids, and other immunosuppressive agents
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Statistical test
- Metastats
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Matched on Factors on which subjects have been matched on in a case-control study
- age, race, sex
Alpha Diversity
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29
Source: Table 1
Description: Differential microbial abundance between papulopustular rosacea (PPR) patients and healthy controls
Abundance in Group 1: increased abundance in Participants with papulopustular rosacea (PPR)
| NCBI | Quality Control | Links |
|---|---|---|
| Alloprevotella tannerae | ||
| Campylobacter ureolyticus | ||
| Corynebacterium kroppenstedtii | ||
| Gleimia europaea | ||
| Prevotella intermedia |
Revision editor(s): Madhubani Dey, Claregrieve1
Signature 2
Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29
Source: Table 1
Description: Differential microbial abundance between papulopustular rosacea (PPR) patients and healthy controls
Abundance in Group 1: decreased abundance in Participants with papulopustular rosacea (PPR)
| NCBI | Quality Control | Links |
|---|---|---|
| Anoxybacillus kestanbolensis | ||
| Azorhizobium doebereinerae | ||
| Cutibacterium granulosum | ||
| Dysgonomonas gadei | ||
| Providencia stuartii | ||
| Shewanella algae |
Revision editor(s): Madhubani Dey, Claregrieve1
