Differential intestinal and oral microbiota features associated with gestational diabetes and maternal inflammation/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/28
Curated date: 2021/06/09
Curator: Madhubani Dey
Revision editor(s): WikiWorks, Madhubani Dey, Claregrieve1, Victoria
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Gestational diabetes diabetes in pregnancy,GDM,gestational diabetes,gestational diabetes mellitus,maternal gestational diabetes mellitus,Gestational diabetes
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Individuals with gestational diabetes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Individuals with gestational diabetes at third trimester during pregnancy
- Group 0 sample size Number of subjects in the control (unexposed) group
- 31
- Group 1 sample size Number of subjects in the case (exposed) group
- 30
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 2 weeks
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- decreased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/28
Source: Figure 2D
Description: Differential microbial abundance between individuals diagnosed with gestational diabetes and healthy controls
Abundance in Group 1: increased abundance in Individuals with gestational diabetes
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacteriaceae | ||
| Bifidobacteriales | ||
| Bifidobacterium | ||
| Selenomonas |
Revision editor(s): Madhubani Dey, Claregrieve1
Signature 2
Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/28
Source: Figure 2D
Description: Differential microbial abundance between individuals diagnosed with gestational diabetes and healthy controls
Abundance in Group 1: decreased abundance in Individuals with gestational diabetes
| NCBI | Quality Control | Links |
|---|---|---|
| Fusobacteriota | ||
| Fusobacteriaceae | ||
| Fusobacteriia | ||
| Lachnoanaerobaculum | ||
| Lactobacillaceae | ||
| Leptotrichia | ||
| Leptotrichiaceae | ||
| Leuconostoc |
Revision editor(s): Madhubani Dey, Claregrieve1
