The impact of NBUVB on microbial community profiling in the lesional skin of vitiligo subjects/Experiment 1
Curated date: 2021/07/13
Curator: Madhubani Dey
Revision editor(s): WikiWorks, Madhubani Dey, Folakunmi
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Skin of body Entire integument,Entire skin,Integument,Integumental organ,Pelt,Skin,Skin organ,Skin of body,skin of body
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Vitiligo VAMAS6,vitiligo,vitiligo-associated multiple autoimmune disease susceptibility 6,Vitiligo
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Vitiligo patients before narrowband UV (NBUVB) light irradiation in normal skin (NF group)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Vitiligo patients after NBUVB irradiation on normal skin (NB group)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Vitiligo patients after NBUVB irradiation on normal skin (NB group); Vitiligo patients recruited from Weifang Medical University Hospital, Shandong, China, from February 2018 to January 2019.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 15
- Group 1 sample size Number of subjects in the case (exposed) group
- 15
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 6 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- increased
Signature 1
Source: within results text (Alterations in the composition of cutaneous microbiota associated with vitiligo at the phylum level, lines 7-9)
Description: Relative abundance of predominant bacteria at phylum and genus level in the NF and NB groups.
Abundance in Group 1: increased abundance in Vitiligo patients after NBUVB irradiation on normal skin (NB group)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidia | ||
| Cyanobacteriota |
Signature 2
Source: within results text (Alterations in the composition of cutaneous microbiota associated with vitiligo at the phylum level, lines 7-9)
Description: Relative abundance of predominant bacteria at phylum and genus level in the NF and NB groups.
Abundance in Group 1: decreased abundance in Vitiligo patients after NBUVB irradiation on normal skin (NB group)
| NCBI | Quality Control | Links |
|---|---|---|
| Actinomycetota | ||
| Pseudomonadota |
Revision editor(s): Folakunmi
