Effects of amoxicillin treatment on the salivary microbiota in children with acute otitis media

From BugSigDB
incomplete
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Lazarevic V, Manzano S, Gaïa N, Girard M, Whiteson K, Hibbs J, François P, Gervaix A, Schrenzel J
Journal
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
Year
2013
Keywords:
Antibiotics, metagenomics, microbiota, otitis media, saliva
Amoxicillin is a first-line antibiotic treatment for acute otitis media in children and one of the most commonly used antibiotics for human bacterial infections. We investigated changes in salivary bacterial communities among children treated with amoxicillin for acute otitis media (n = 18), using a culture-independent approach based on pyrosequencing of the V3 region of the bacterial 16S rRNA gene. The control group consisted of children with acute otitis media who were not given antibiotics (n = 15). One species-level phylotype assigned to the genus Streptococcus was identified across all (n = 99) saliva samples. Two additional species-level phylotypes from the genera Gemella and Granulicatella were shared by all (n = 45) samples of control subjects. Amoxicillin treatment resulted in reduced species richness and diversity, and a significant shift in the relative abundance of 35 taxa at different ranks from phylum to species-level phylotype. At the phylum level, prevalence of TM7 and Actinobacteria decreased at the end of treatment, whereas Proteobacteria had a higher relative abundance post-treatment. Multivariate analysis showed that samples from the same control subject taken over time intervals tended to cluster together. Among antibiotic-treated subjects, samples taken before and at the end of amoxicillin treatment formed two relatively well-separated clusters both of which greatly overlapped with samples taken about 3 weeks post-treatment. Our results point to a substantial but incomplete recovery of the salivary bacterial community from the antibiotic about 3 weeks after the end of treatment.

Experiment 1


Needs review

Curated date: 2021/07/19

Curator: Gina

Revision editor(s): Chloe, Gina, Lwaldron, WikiWorks

Subjects

Location of subjects
Switzerland
Host species Species from which microbiome was sampled (if applicable)
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
otitis media with effusion Glue ear,Mucoid otitis media,Mucoid otitis media NOS (disorder),OME,Otitis Media with Effusion,Otitis media with effusion,otitis media with effusion,Otitis media with effusion - mucoid,Otitis media, transudative,Secretory Otitis Media,secretory otitis Media,serous otitis Media,Transudative otitis media
Group 0 name Corresponds to the control (unexposed) group for case-control studies
not treated with amoxicillin (end of treatment)
Group 1 name Corresponds to the case (exposed) group for case-control studies
treated with amoxicillin (end of treatment)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
The amoxicillin group was patients/children aged from 12 to 72 months with clinically diagnosed AOM whose care providers prescribed amoxicillin treatment. Excluded criteria contained pharyngitis.
Group 0 sample size Number of subjects in the control (unexposed) group
15
Group 1 sample size Number of subjects in the case (exposed) group
18
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
6 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V1-V3
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Roche454

Statistical Analysis

Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
Matched on Factors on which subjects have been matched on in a case-control study
antimicrobial agent, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Richness Number of species
increased

Experiment 2


incomplete

Curated date: 2021/07/20

Curator: Chloe

Revision editor(s): Chloe

Differences from previous experiment shown

Subjects

Location of subjects
Not specified
Host species Species from which microbiome was sampled (if applicable)
Not specified
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Not specified
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Not specified
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Pre-amoxicillin treatment
Group 1 name Corresponds to the case (exposed) group for case-control studies
End-of-treatment
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Not specified
Group 0 sample size Number of subjects in the control (unexposed) group
Not specified
Group 1 sample size Number of subjects in the case (exposed) group
Not specified
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
Not specified
Sequencing type
Not specified
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Not specified
Statistical test
Not specified
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
Not specified
Matched on Factors on which subjects have been matched on in a case-control study
Not specified


Signature 1

Needs review

Curated date: 2021/07/20

Curator: Chloe

Revision editor(s): Chloe, Lwaldron

Source: Table 2

Description:

Abundance in Group 1: decreased abundance in End-of-treatment

NCBI Quality ControlLinks
Actinomycetota
Candidatus Saccharibacteria

Revision editor(s): Chloe, Lwaldron