Gut microbiota imbalance and its correlations with hormone and inflammatory factors in patients with stage 3/4 endometriosis

Study information

Reviewed Marked as Reviewed by Folakunmi on 2024-2-20

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

33839907

DOI Digital object identifier for electronic documents.

10.1007/s00404-021-06057-z

URI

Authors

Shan J, Ni Z, Cheng W, Zhou L, Zhai D, Sun S, Yu C

Journal

Archives of gynecology and obstetrics

Year

2021

Keywords:

Dysbiosis, Endometriosis, Estradiol, Gut microbiota, Inflammatory factors

Endometriosis (EM) in reproductive females has an incidence of 6-10% and greatly affects female fertility, quality of life, and long-term health. The gut microbiota can affect the physiological and pathological processes of humans through various pathways, such as those involving the nervous and endocrine systems and immunity, and it plays important roles in endocrine and inflammatory diseases. Whether the gut microbiota plays a role in EM has gradually attracted researchers' attention. In the present study, fecal and blood samples were collected from 12 patients with stage 3/4 EM and 12 healthy controls. We performed 16S rRNA high-throughput sequencing to compare the gut microbiota between the EM and control groups. Serum levels of hormones and inflammatory cytokines were measured. We found that compared with the control group, the EM group had a lower α diversity of gut microbiota and a higher Firmicutes/Bacteroidetes ratio. The abundances of various taxa (such as Actinobacteria, Tenericutes, Blautia, Bifidobacterium, Dorea, and Streptococcus) were significantly different between the two groups. The taxon with the highest abundance in the EM group was Prevotella_7, and that in the control group was Coprococcus_2. The serum levels of E2 and IL-8 were significantly higher in the EM group than in the control group (E2: EM group 74.7 ± 22.5 pg/L vs CON group 47.9 ± 12.5 pg/L; IL-8: EM group 6.39 ± 1.59 pg/mL vs CON group 4.14 ± 0.73 pg/mL). Additionally, the gut microbiota of the EM group was enriched for the microbial function categories environmental information processing, endocrine system, and immune system. Correlations were detected between each of Blautia and Dorea abundance and estradiol level and between Subdoligranulum abundance and IL-8 level. This study elucidated the associations between the gut microbiota and both serum hormones and inflammatory factors in EM. However, the findings need to be verified in future studies.

Experiment 1

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Reviewed Marked as Reviewed by Folakunmi on 2024-2-20

Curated date: 2021/08/09

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Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: Stage 3/4 endometriosis patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Women were diagnosed with endometriosis via laparoscopic surgery and stages were categorized according to the revised American Society for Reproductive Medicine scoring system (r-ASRM).

Group 0 sample size Number of subjects in the control (unexposed) group: 12

Group 1 sample size Number of subjects in the case (exposed) group: 12

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 3 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Richness Number of species: unchanged

Signature 1

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Curated date: 2021/08/09

Curator: Samara.Khan

Revision editor(s): WikiWorks, Samara.Khan, Folakunmi, Idiaru angela, Welile

Source: Figure 3

Description: Patients with endometriosis demonstrated a significantly higher abundance of the following taxa compared to control patients. There were 36 taxa unique to endometriosis patients that were completely absent in control patients. Some taxa were excluded because they had an unidentified genus/ species

Abundance in Group 1: increased abundance in

NCBI	Quality Control	Links
Acidobacteriota
Actinomyces
Anaerobutyricum hallii
Bacteroidales
Blautia
Candidatus Saccharibacteria
Cetobacterium
Corynebacterium
Cyanobacteriota
Dorea
Eggerthella
Enterococcus
Granulicatella
Lactobacillus
Lactococcus
Mediterraneibacter gnavus
Mycobacterium
Pseudarthrobacter
Ruminiclostridium
Streptococcus
[Eubacterium] brachy
unclassified Blastocatellaceae
unclassified Prevotellaceae
unclassified Rhodobiaceae
Clostridium

Revision editor(s): WikiWorks, Samara.Khan, Folakunmi, Idiaru angela, Welile

Signature 2

Edit History Delete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2021/08/09

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Folakunmi, WikiWorks

Source: Figure 3

Description: The following taxa were decreased in endometriosis patients and increased in the control group. There were 11 genera unique to the control group. Some taxa were excluded because they had an unidentified genus/ species

Abundance in Group 1: decreased abundance in

NCBI	Quality Control	Links
Lachnospira
Lachnospira eligens
Lachnospiraceae bacterium NK4A136
Odoribacter
Parasutterella
Ruminococcus sp.
bacterium ND3007
unclassified Defluviitaleaceae
unclassified Ruminiclostridium
uncultured Oscillospiraceae bacterium
mollicutes_RF9mollicutes_RF9
uncultured Lachnospiraceae bacterium

Revision editor(s): Samara.Khan, Folakunmi, WikiWorks