Antibiotic therapy with metronidazole reduces endometriosis disease progression in mice: a potential role for gut microbiota/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/8

Curated date: 2021/08/09

Curator: Samara.Khan

Revision editor(s): WikiWorks, Claregrieve1, Samara.Khan, Peace Sandy

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Endometriosis endometriosis,Endometriosis (clinical),endometriosis (disease),Endometriosis (disorder),Endometriosis (morphologic abnormality),ENDOMETRIOSIS NEC,Endometriosis NOS,Endometriosis NOS (disorder),Endometriosis of other specified sites,Endometriosis, site unspecified,Endometriosis
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Non-endo mice
Group 1 name Corresponds to the case (exposed) group for case-control studies
Endo mice
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Uterine tissue was transplanted onto the peritoneal wall in order to induce endometriosis in these mice.
Group 0 sample size Number of subjects in the control (unexposed) group
5
Group 1 sample size Number of subjects in the case (exposed) group
5
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
Mice had never been given antibiotics prior to the start of the experiment

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/8

Curated date: 2021/08/09

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1

Source: Figure 3

Description: Differential microbial abundance between non-endo and endo mice: fecal samples from mice with simulated endometriosis had higher levels of Bacteroidetes and Firmicutes than non-endo mice. The bacteroides genera was absent in non-endo mice but present in endo mice.

Abundance in Group 1: increased abundance in Endo mice

NCBI Quality ControlLinks
Bacteroides

Revision editor(s): Samara.Khan, Claregrieve1