The Vaginal Microbiome as a Tool to Predict rASRM Stage of Disease in Endometriosis: a Pilot Study/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-22
Subjects
- Location of subjects
- Brazil
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Vagina Vagina,vagina,Distal oviductal region,Distal portion of oviduct,Vaginae
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Endometriosis endometriosis,Endometriosis (clinical),endometriosis (disease),Endometriosis (disorder),Endometriosis (morphologic abnormality),ENDOMETRIOSIS NEC,Endometriosis NOS,Endometriosis NOS (disorder),Endometriosis of other specified sites,Endometriosis, site unspecified,Endometriosis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- patients with rASRM stages 1 - 2
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- patients with rASRM stages 3 - 4
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients with vaginal and rectal microbiome profiles and their association to severity of endometriosis (rASRM) stages 3 - 4
- Group 0 sample size Number of subjects in the control (unexposed) group
- 12
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Participants were excluded if they had taken antibiotics within the past 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-22
Source: Fig 3B
Description: Correlations of OTU abundance with stage of disease, showing abundance in stages 3 - 4 compared to stages 1 - 2
Abundance in Group 1: decreased abundance in patients with rASRM stages 3 - 4
| NCBI | Quality Control | Links |
|---|---|---|
| Haemophilus | ||
| Neisseria | ||
| Porphyromonas | ||
| Streptococcus |
Revision editor(s): Atrayees, ChiomaBlessing
Signature 2
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-22
Source: Fig 3B
Description: Correlations of OTU abundance with stage of disease, showing abundance in stages 3 - 4 compared to stages 1 - 2
Abundance in Group 1: increased abundance in patients with rASRM stages 3 - 4
Revision editor(s): Atrayees, ChiomaBlessing
