Exercise Alters Gut Microbiota Composition and Function in Lean and Obese Humans/Experiment 2

From BugSigDB


Reviewed Marked as Reviewed by Claregrieve1 on 2022/12/29

Curated date: 2022/12/29

Curator: Claregrieve1

Revision editor(s): Claregrieve1, WikiWorks

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Exercise Exercise,exercise
Group 0 name Corresponds to the control (unexposed) group for case-control studies
lean subjects
Group 1 name Corresponds to the case (exposed) group for case-control studies
obese subjects
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
sedentary participants between 20 and 45 years of age with BMI >30 (classified as obese)
Group 0 sample size Number of subjects in the control (unexposed) group
18
Group 1 sample size Number of subjects in the case (exposed) group
14
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
PERMANOVA
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/12/29

Curated date: 2022/12/29

Curator: Claregrieve1

Revision editor(s): Claregrieve1, Lwaldron

Source: Figure 2d

Description: Differential abundance between obese and lean participants after the 6 week sedentary washout post-exercise program

Abundance in Group 1: decreased abundance in obese subjects

NCBI Quality ControlLinks
Dorea
Collinsella

Revision editor(s): Claregrieve1, Lwaldron

Signature 2

Needs review

Curated date: 2023/03/16

Curator: Lwaldron

Revision editor(s): Lwaldron

Source: Figure 2e

Description: Differential abundance between obese and lean participants after the 6 week sedentary washout post-exercise program

Abundance in Group 1: increased abundance in obese subjects

NCBI Quality ControlLinks
Faecalibacterium
Lachnospira

Revision editor(s): Lwaldron