Fiber-Mediated Nourishment of Gut Microbiota Protects against Diet-Induced Obesity by Restoring IL-22-Mediated Colonic Health/Experiment 1
Curated date: 2021/10/06
Curator: Lorakasselman
Revision editor(s): WikiWorks, LGeistlinger, Lorakasselman, Atrayees, Aiyshaaaa, Peace Sandy
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- High fat diet HF - High fat diet,High fat diet (finding),High fat diet,high fat diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- High fat diet (HFD: 50 cell)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- High fat diet (HFD:200 Inul )
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- High fat diet - [HFD] enriched with inulin increased gut epithelial proliferation, prevented colon atrophy
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Curated date: 2021/10/06
Curator: Lorakasselman
Revision editor(s): Lorakasselman, Aiyshaaaa, Merit, Peace Sandy
Source: Figure S3D
Description: Taxonomic cladogram obtained from LEfSe analysis of 16S sequences by comparing HFD enrichment of inulin with standard HFD (50g cellulose)
Abundance in Group 1: increased abundance in High fat diet (HFD:200 Inul )
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacteriaceae | ||
| Bifidobacteriales | ||
| Bifidobacterium | ||
| Coriobacteriaceae | ||
| Coriobacteriales | ||
| Rikenellaceae |
Revision editor(s): Lorakasselman, Aiyshaaaa, Merit, Peace Sandy
Signature 2
Curated date: 2021/10/07
Curator: Chloe
Revision editor(s): Chloe, Lorakasselman, Aiyshaaaa, Peace Sandy
Source: Figure S3D
Description: Taxonomic cladogram obtained from LEfSe analysis of 16S sequences by comparing HFD enrichment of Inulin with standard HFD
Abundance in Group 1: decreased abundance in High fat diet (HFD:200 Inul )
Revision editor(s): Chloe, Lorakasselman, Aiyshaaaa, Peace Sandy
