Effect of electronic cigarette and tobacco smoking on the human saliva microbial community/Experiment 2

From BugSigDB


Reviewed Marked as Reviewed by Atrayees on 2023-6-21

Curated date: 2023/06/06

Curator: Aiyshaaaa

Revision editor(s): Aiyshaaaa, Atrayees

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Smoking behavior smoking,Smoking behavior,smoking behavior
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Non Smokers
Group 1 name Corresponds to the case (exposed) group for case-control studies
E-cigarette smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
The volunteers who smoked electronic cigarettes.
Group 0 sample size Number of subjects in the control (unexposed) group
6
Group 1 sample size Number of subjects in the case (exposed) group
5
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
6 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4-V5
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Ion Torrent

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
ANOVA
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 2

Reviewed Marked as Reviewed by Atrayees on 2023-6-21

Curated date: 2023/06/06

Curator: Aiyshaaaa

Revision editor(s): Aiyshaaaa

Source: Figure 4 (C-F)

Description: The relative abundances of Neisseria, Prevotellaceae, Corynebacterium, and Porphyromonas in the two groups.

Abundance in Group 1: decreased abundance in E-cigarette smokers

NCBI Quality ControlLinks
Neisseria
Corynebacterium

Revision editor(s): Aiyshaaaa