Effect of electronic cigarette and tobacco smoking on the human saliva microbial community/Experiment 3
From BugSigDB
Reviewed Marked as Reviewed by Atrayees on 2023-6-21
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Smoking behavior smoking,Smoking behavior,smoking behavior
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Tobacco smokers
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- E-cigarette smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The volunteers who smoked electronic cigarettes.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 14
- Group 1 sample size Number of subjects in the case (exposed) group
- 5
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 6 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4-V5
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Ion Torrent
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- ANOVA
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-6-21
Source: Figure 4 (C-F)
Description: The relative abundances of Neisseria, Prevotellaceae, Corynebacterium, and Porphyromonas in the two groups.
Abundance in Group 1: decreased abundance in E-cigarette smokers
| NCBI | Quality Control | Links |
|---|---|---|
| Neisseria |
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-6-21
Source: Figure 4 (C-F)
Description: The relative abundances of Neisseria, Prevotellaceae, Corynebacterium, and Porphyromonas in the two groups.
Abundance in Group 1: increased abundance in E-cigarette smokers
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotellaceae |
