Effect of ethnicity and socioeconomic variation to the gut microbiota composition among pre-adolescent in Malaysia/Experiment 1

Reviewed Marked as Reviewed by Fatima on 2022/07/6

Curated date: 2022/06/24

Curator: Kaluifeanyi101

Revision editor(s): Fatima, Claregrieve1, Kaluifeanyi101, Peace Sandy

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Socioeconomic status class,Socioeconomic status,socioeconomic status,socioeconomic factors

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Chinese Children (High SES, household income>RM500)

Group 1 name Corresponds to the case (exposed) group for case-control studies: Malays Children (Moderate SES. house income=<RM500)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Gut microbiota from fecal samples of 24 Malays children whose household income =<RM500. Note: Poverty line = RM 764($254).

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): unspecified

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V5

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa: unchanged