Subgingival microbiome of rheumatoid arthritis patients in relation to their disease status and periodontal health/Experiment 2
Subjects
- Location of subjects
- Norway
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Rheumatoid arthritis Arthritis or polyarthritis, rheumatic,arthritis or polyarthritis, rheumatic,Arthritis, Rheumatoid,arthritis, rheumatoid,atrophic Arthritis,atrophic arthritis,autoimmune arthritis,Chronic rheumatic arthritis,Proliferative arthritis,RA,RA - Rheumatoid arthritis,RhA - Rheumatoid arthritis,Rheumatic gout,rheumatoid arthritis,Rheumatoid arthritis (disorder),Rheumatoid arthritis NOS,Rheumatoid arthritis NOS (disorder),rheumatoid arthritis, susceptibility to,Rheumatoid disease,Rheumatoid arthritis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- No prednisolone
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Prednisolone
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Individuals who received prednisolone
- Group 0 sample size Number of subjects in the control (unexposed) group
- 57
- Group 1 sample size Number of subjects in the case (exposed) group
- 21
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Source: S4F Table
Description: Significantly discriminatory OTUs between the microbiome sample clusters (use - and non-use of prednisolone). From the 552 OTUs, 19 discriminated significantly use and non-use of prednisolone (LDA >2, p<0.05, LEfSe). Prednisolone (N=21)=black dots; no prednisolone (N=57)=grey dots.
Abundance in Group 1: increased abundance in Prednisolone
| NCBI | Quality Control | Links |
|---|---|---|
| Kingella | ||
| Capnocytophaga | ||
| Streptococcus | ||
| Cardiobacterium | ||
| Selenomonas |
Revision editor(s): Peace Sandy
Signature 2
Source: S4F Table
Description: Significantly discriminatory OTUs between the microbiome sample clusters (use - and non-use of prednisolone). From the 552 OTUs, 19 discriminated significantly use and non-use of prednisolone (LDA >2, p<0.05, LEfSe). Prednisolone (N=21)=black dots; no prednisolone (N=57)=grey dots.
Abundance in Group 1: decreased abundance in Prednisolone
| NCBI | Quality Control | Links |
|---|---|---|
| Atopobium | ||
| Clostridiales Family XIII bacterium | ||
| Coriobacteriaceae | ||
| Defluviitaleaceae | ||
| Dialister | ||
| Fretibacterium | ||
| Fusobacterium | ||
| Mogibacterium | ||
| Prevotella | ||
| Treponema |
Revision editor(s): Peace Sandy
