Biliary Microbiota in Choledocholithiasis and Correlation With Duodenal Microbiota

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Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-18
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Han J, Wu S, Fan Y, Tian Y, Kong J
Journal
Frontiers in cellular and infection microbiology
Year
2021
Keywords:
antimicrobial resistance, biliary microbiota, choledocholithiasis, duodenal microbiota, duodenal–biliary reflux
BACKGROUND: The pathogenesis of choledocholithiasis is closely related to the role of bacteria. However, little is known about the predictive role of bile bacteria in clinical conditions of patients and the compositional and functional characteristics of biliary microbiota in choledocholithiasis. METHODS: To investigate the predictive value of biliary bacteria, clinical data of 488 patients with choledocholithiasis were collected. The predictive value of common bile bacteria to patients' clinical conditions was analyzed by logistic regression. Samples of bile and corresponding duodenal juice from 10 selected patients with choledocholithiasis were obtained, and the composition and function of microbial communities were analyzed based on 16S rRNA sequencing and Tax4Fun. RESULTS: The clinical conditions of patients with choledocholithiasis, such as recurrence, the severity of acute cholangitis, and duration of hospital stay were closely related to different species of bile bacteria as well as antimicrobial-resistant bacteria. Employing 16S rRNA sequencing, the dominant phyla of biliary and duodenal microbiota were Proteobacteria and Firmicutes. The top three core microbiota at the genus level were Escherichia-Shigella, Fusobacterium, and Enterococcus. Escherichia coli accounted for the most abundant annotated species in both. Differences in composition between biliary and duodenal microbiota were not significant according to the alpha and beta diversities. Differential abundant features were not found in biliary microbiota indicated by A linear discriminant analysis effective size algorithm. The major pathways identified in biliary and duodenal microbiota were related to membrane transport, translation, replication and repair, carbohydrate and amino acid metabolism. However, no significant difference in those major pathways, as well as antimicrobial-resistance patterns, was observed between biliary and duodenal microbiota. CONCLUSION: Our study first demonstrates the predictive contribution of biliary bacteria to the clinical conditions of patients with choledocholithiasis, and then it offers new insights into the compositional and functional features of biliary and duodenal microbiota. Similarities between biliary and duodenal microbiota support the theory of bacterial duodenal-biliary reflux in patients with choledocholithiasis. Meanwhile, when it is impracticable to obtain a bile sample, duodenal juice may be used as an alternative for bacterial culture and susceptibility tests.

Experiment 1


Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-18

Curated date: 2023/12/14

Curator: Folakunmi

Revision editor(s): Folakunmi

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Duodenum , Bile duct Proximal intestine,Upper intestine,Duodenum,duodenum,Bile tube,Biliary duct,Gall duct,Hepatic duct,Bile duct,bile duct
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Sampling site Sampling site,sampling site
Group 0 name Corresponds to the control (unexposed) group for case-control studies
biliary microbiota
Group 1 name Corresponds to the case (exposed) group for case-control studies
duodenal microbiota
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
duodenal juice samples from patients with choledocholithiasis
Group 0 sample size Number of subjects in the control (unexposed) group
10
Group 1 sample size Number of subjects in the case (exposed) group
10
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
4 weeks

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
4

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-18

Curated date: 2023/12/14

Curator: Folakunmi

Revision editor(s): Folakunmi

Source: figure 2D

Description: microbial taxa characterizing duodenal juice from bile in patients with choledocholithiasis as determined by LEfSe

Abundance in Group 1: increased abundance in duodenal microbiota

NCBI Quality ControlLinks
Actinomycetota
Bacilli
Lactobacillales
Streptococcaceae
Streptococcus
unclassified Actinomycetota

Revision editor(s): Folakunmi