Salivary microbiome in children with Down syndrome: a case-control study/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by Folakunmi on 2024-1-10
Subjects
- Location of subjects
- Japan
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Oral cavity Bucca,Buccal cavity,Cavity of mouth,Oral cavity,oral cavity
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Down syndrome Complete trisomy 21 syndrome,complete trisomy 21 syndrome,Complete trisomy 21 syndrome (disorder),Down syndrome,Down syndrome chromosome region,Down syndrome critical region,Down Syndrome, Partial Trisomy 21,DOWN'S SYNDROME,Down's syndrome,Down's syndrome - trisomy 21,Down's syndrome NOS,Down's syndrome NOS (disorder),Downs Syndrome,Downs syndrome,G Trisomy,G trisomy,leukemia, megakaryoblastic, of Down syndrome,Mongolism,Partial Trisomy 21 Down Syndrome,Syndrome, Down,Syndrome, Down's,T21 - Trisomy 21,transient myeloproliferative disorder of Down syndrome,Trisomy 21,trisomy 21,trisomy 21 (Down syndrome),Trisomy 21 (Down Syndrome)Downs SyndromeTrisomy 21 Syndrome,Trisomy 21 NOS,trisomy 21 syndrome,Trisomy 21, Meiotic Nondisjunction,Trisomy 21, Mitotic Nondisjunction,down syndrome
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Controls children with mixed dentition (PD)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Down Syndrome children with mixed dentition (PD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Children with Down Syndrome and in the mixed dentition stage (from 6 years to around 12 years old)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 15
- Group 1 sample size Number of subjects in the case (exposed) group
- 15
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 7 days
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V2
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- Matched on Factors on which subjects have been matched on in a case-control study
- age
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-10
Source: Figure 5
Description: Species level of salivary microbiomes between children with DS and ND (MD stage)
Abundance in Group 1: increased abundance in Down Syndrome children with mixed dentition (PD)
Revision editor(s): Andre
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-1-10
Source: Figure 5
Description: Species-level of salivary microbiomes between children with DS and ND (MD stage)
Abundance in Group 1: decreased abundance in Down Syndrome children with mixed dentition (PD)
Revision editor(s): Andre
