The oral microbiome and breast cancer and nonmalignant breast disease, and its relationship with the fecal microbiome in the Ghana Breast Health Study

Study information

Needs review

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

35657343

DOI Digital object identifier for electronic documents.

10.1002/ijc.34145

URI

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420782/

Authors

Wu Z, Byrd DA, Wan Y, Ansong D, Clegg-Lamptey JN, Wiafe-Addai B, Edusei L, Adjei E, Titiloye N, Dedey F, Aitpillah F, Oppong J, Vanderpuye V, Osei-Bonsu E, Dagnall CL, Jones K, Hutchinson A, Hicks BD, Ahearn TU, Shi J, Knight R, Biritwum R, Yarney J, Wiafe S, Awuah B, Nyarko K, Figueroa JD, Sinha R, Garcia-Closas M, Brinton LA, Vogtmann E

Journal

International journal of cancer

Year

2022

Keywords:

Ghana, breast cancer, fecal microbiome, nonmalignant breast diseases, oral microbiome

The oral microbiome, like the fecal microbiome, may be related to breast cancer risk. Therefore, we investigated whether the oral microbiome was associated with breast cancer and nonmalignant breast disease, and its relationship with the fecal microbiome in a case-control study in Ghana. A total of 881 women were included (369 breast cancers, 93 nonmalignant cases and 419 population-based controls). The V4 region of the 16S rRNA gene was sequenced from oral and fecal samples. Alpha-diversity (observed amplicon sequence variants [ASVs], Shannon index and Faith's Phylogenetic Diversity) and beta-diversity (Bray-Curtis, Jaccard and weighted and unweighted UniFrac) metrics were computed. MiRKAT and logistic regression models were used to investigate the case-control associations. Oral sample alpha-diversity was inversely associated with breast cancer and nonmalignant breast disease with odds ratios (95% CIs) per every 10 observed ASVs of 0.86 (0.83-0.89) and 0.79 (0.73-0.85), respectively, compared to controls. Beta-diversity was also associated with breast cancer and nonmalignant breast disease compared to controls (P ≤ .001). The relative abundances of Porphyromonas and Fusobacterium were lower for breast cancer cases compared to controls. Alpha-diversity and presence/relative abundance of specific genera from the oral and fecal microbiome were strongly correlated among breast cancer cases, but weakly correlated among controls. Particularly, the relative abundance of oral Porphyromonas was strongly, inversely correlated with fecal Bacteroides among breast cancer cases (r = -.37, P ≤ .001). Many oral microbial metrics were strongly associated with breast cancer and nonmalignant breast disease, and strongly correlated with fecal microbiome among breast cancer cases, but not controls.

Experiment 1

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Curated date: 2024/03/07

Curator: Muqtadirat

Revision editor(s): Muqtadirat

Subjects

Location of subjects: Ghana

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Breast cancer breast cancer,breast tumor,cancer of breast,malignant breast neoplasm,malignant breast tumor,malignant neoplasm of breast,malignant neoplasm of the breast,malignant tumor of breast,malignant tumor of the breast,mammary cancer,mammary neoplasm,mammary tumor,primary breast cancer,Breast cancer

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Control

Group 1 name Corresponds to the case (exposed) group for case-control studies: Breast Health Condition

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Women with breast cancer cases

Group 0 sample size Number of subjects in the control (unexposed) group: 419

Group 1 sample size Number of subjects in the case (exposed) group: 369

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 1 month

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Logistic Regression

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Matched on Factors on which subjects have been matched on in a case-control study: age, geographic area

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment: age, alcohol drinking, body mass index, contraception, family history of cancer, antibiotic exposure, education level, geographic area, smoking status

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Richness Number of species: increased

Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample: increased

Signature 1

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Curated date: 2024/03/09

Curator: Muqtadirat

Revision editor(s): Muqtadirat, MyleeeA

Source: Suppl. Table S1

Description: Genera with higher odds ratio in breast cancer cases in comparison with control

Abundance in Group 1: increased abundance in Breast Health Condition

NCBI	Quality Control	Links
Murdochiella
Parvimonas
Porphyromonas
Prevotella
Scardovia
Tannerella
unclassified Prevotellaceae
Prevotella sp. 1-8

Revision editor(s): Muqtadirat, MyleeeA

Experiment 2

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Subjects

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Women with non malignant cases

Group 1 sample size Number of subjects in the case (exposed) group: 93

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Richness Number of species: increased

Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample: increased

Signature 1

Edit History Delete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2024/03/10

Curator: Muqtadirat

Revision editor(s): Muqtadirat, MyleeeA

Source: Suppl. Table S1

Description: Genera with higher odds ratio in non malignant cases in comparison with control

Abundance in Group 1: increased abundance in Breast Health Condition

NCBI	Quality Control	Links
Bergeyella
Mogibacterium
Porphyromonas
Prevotella
Scardovia
Tannerella
unclassified Prevotellaceae
Prevotella sp. 1-8

Revision editor(s): Muqtadirat, MyleeeA

Experiment 3

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Your review change was submittedDuplicate this Experiment Add a new Signature

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Curated date: 2024/03/10

Curator: Muqtadirat

Revision editor(s): Muqtadirat

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Non malignant

Group 1 name Corresponds to the case (exposed) group for case-control studies: Breast cancer

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Women with breast cancer cases