The Influence of Age and Sex on Ocular Surface Microbiota in Healthy Adults/Experiment 2
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Bulbar conjunctiva Ocular conjunctiva,Bulbar conjunctiva,bulbar conjunctiva
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Biological sex Biological sex,biological sex
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Male
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Female
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Biological sex=female
- Group 0 sample size Number of subjects in the control (unexposed) group
- 42
- Group 1 sample size Number of subjects in the case (exposed) group
- 48
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- No history of antibiotics in the past 6 months
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 3
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Source: Figure 4a
Description: FIGURE 4. Sex-differences in conjunctival microbiome of healthy adults. Male and female groups,... while no significant difference is observed in Shannon diversity index (A). (Note- no taxon were found to be decreased in females compared to males)
Abundance in Group 1: increased abundance in Female
| NCBI | Quality Control | Links |
|---|---|---|
| Escherichia coli | ||
| Micrococcus luteus | ||
| [Acidovorax] ebreus | ||
| Staphylococcus haemolyticus | ||
| Acinetobacter baumannii |
Revision editor(s): Mary Bearkland, Peace Sandy
Signature 2
Source: Figure 4a
Description: FIGURE 4. Sex-differences in conjunctival microbiome of healthy adults. Male and female groups,... while no significant difference is observed in Shannon diversity index (A). (Note- no taxon were found to be increased in females compared to males)
Abundance in Group 1: decreased abundance in Female
| NCBI | Quality Control | Links |
|---|---|---|
| Cutibacterium acnes | ||
| Staphylococcus epidermidis |
Revision editor(s): Mary Bearkland, Peace Sandy
