Dynamic gut microbiome across life history of the malaria mosquito Anopheles gambiae in Kenya/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by Peace Sandy on 2024-2-20
Subjects
- Location of subjects
- Kenya
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Anopheles gambiae
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Instar larval stage , Pupa Instar larval stage,instar larval stage,Aurelia,Chrysalides,Chrysalis,Pupae,Pupa,pupa
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Gut microbiome measurement Gut microbiome measurement,gut microbiome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Sugar fed mosquito - 3-day-old,sugar (triplicates)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Blood fed mosquito - 2 days post blood meal (triplicates)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Blood-fed mosquito at different time points
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- NIL
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V3
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Roche454
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- Metastats
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
Signature 1
Reviewed Marked as Reviewed by Peace Sandy on 2024-2-20
Source: Table S7 and Table S6
Description: Differentially abundant genera before and after a blood meal
Differentially abundant families before and after a blood meal
Abundance in Group 1: increased abundance in Blood fed mosquito - 2 days post blood meal (triplicates)
| NCBI | Quality Control | Links |
|---|---|---|
| Enterobacter | ||
| Elizabethkingia | ||
| Enterobacteriaceae | ||
| Aeromonadaceae | ||
| Pseudomonadaceae |
Revision editor(s): Peace Sandy
Signature 2
Reviewed Marked as Reviewed by Peace Sandy on 2024-2-20
Source: Table S7 and Table S6
Description: Differentially abundant genera before and after a blood meal
Differentially abundant families before and after a blood meal
Abundance in Group 1: decreased abundance in Blood fed mosquito - 2 days post blood meal (triplicates)
| NCBI | Quality Control | Links |
|---|---|---|
| Comamonadaceae | ||
| Flavobacteriaceae | ||
| Moraxellaceae | ||
| Propionibacteriaceae | ||
| Raoultella | ||
| Sphingomonadaceae | ||
| Lysobacteraceae |
Revision editor(s): Peace Sandy
