Effects of surgical and dietary weight loss therapy for obesity on gut microbiota composition and nutrient absorption/Experiment 2

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Subjects

Location of subjects
Germany
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity
Group 0 name Corresponds to the control (unexposed) group for case-control studies
very low calorie diet after 3 months and 6 months
Group 1 name Corresponds to the case (exposed) group for case-control studies
Very low calorie diet baseline
Group 0 sample size Number of subjects in the control (unexposed) group
3
Group 1 sample size Number of subjects in the case (exposed) group
3
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Roche454

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Signature 1

Reviewed Marked as Reviewed by Fatima on 2021/08/9

Curated date: 2021/01/10

Curator: Marianthi Thomatos

Revision editor(s): Fatima, WikiWorks

Source: Table S1B

Description: Effects of laparoscopic sleeve gastrectomy (LSG) and very low calorie diet (VLCD) for obesity on gut microbiota composition

Abundance in Group 1: decreased abundance in Very low calorie diet baseline

NCBI Quality ControlLinks
Bacilli
Blautia hydrogenotrophica
Clostridium sp. D5
Clostridium sp. M62/1
Coprobacillus
Enterococcaceae
Enterococcus
Enterococcus faecium
Eubacterium limosum
Fusobacteriota
Fusobacteriaceae
Fusobacteriales
Holdemania
Lacrimispora saccharolytica
Lactobacillales
Lactobacillus
Oribacterium
Oscillospiraceae
Peptostreptococcaceae
Ruminococcus
[Clostridium] symbiosum
Coprobacillus cateniformis

Revision editor(s): Fatima, WikiWorks