Lower respiratory tract microbiome composition and community interactions in smokers

From BugSigDB
Reviewed Marked as Reviewed by Folakunmi on 2024-2-16
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Campos M, Cickovski T, Fernandez M, Jaric M, Wanner A, Holt G, Donna E, Mendes E, Silva-Herzog E, Schneper L, Segal J, Amador DM, Riveros JD, Aguiar-Pulido V, Banerjee S, Salathe M, Mathee K, Narasimhan G
Journal
Access microbiology
Year
2023
Keywords:
clustering, co-occurrence networks, lower respiratory tract (LRT), microbiome, smoking
The lung microbiome impacts on lung function, making any smoking-induced changes in the lung microbiome potentially significant. The complex co-occurrence and co-avoidance patterns between the bacterial taxa in the lower respiratory tract (LRT) microbiome were explored for a cohort of active (AS), former (FS) and never (NS) smokers. Bronchoalveolar lavages (BALs) were collected from 55 volunteer subjects (9 NS, 24 FS and 22 AS). The LRT microbiome composition was assessed using 16S rRNA amplicon sequencing. Identification of differentially abundant taxa and co-occurrence patterns, discriminant analysis and biomarker inferences were performed. The data show that smoking results in a loss in the diversity of the LRT microbiome, change in the co-occurrence patterns and a weakening of the tight community structure present in healthy microbiomes. The increased abundance of the genus Ralstonia in the lung microbiomes of both former and active smokers is significant. Partial least square discriminant and DESeq2 analyses suggested a compositional difference between the cohorts in the LRT microbiome. The groups were sufficiently distinct from each other to suggest that cessation of smoking may not be sufficient for the lung microbiota to return to a similar composition to that of NS. The linear discriminant analysis effect size (LEfSe) analyses identified several bacterial taxa as potential biomarkers of smoking status. Network-based clustering analysis highlighted different co-occurring and co-avoiding microbial taxa in the three groups. The analysis found a cluster of bacterial taxa that co-occur in smokers and non-smokers alike. The clusters exhibited tighter and more significant associations in NS compared to FS and AS. Higher degree of rivalry between clusters was observed in the AS. The groups were sufficiently distinct from each other to suggest that cessation of smoking may not be sufficient for the lung microbiota to return to a similar composition to that of NS.

Experiment 1


Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2023/10/08

Curator: OdigiriGreat

Revision editor(s): OdigiriGreat

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Lung Pulmo,Lung,lung
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Smoking status measurement Smoking status measurement,smoking status measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Never smokers
Group 1 name Corresponds to the case (exposed) group for case-control studies
Active smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Participants who have smoked at least one cigarette within 3days of enrolment
Group 0 sample size Number of subjects in the control (unexposed) group
9
Group 1 sample size Number of subjects in the case (exposed) group
22
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
use of antibiotics within 12weeks prior to study

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V6-V8
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Roche454

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Chao1 Abundance-based estimator of species richness
decreased
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
decreased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
decreased

Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2023/10/08

Curator: OdigiriGreat

Revision editor(s): OdigiriGreat, Folakunmi

Source: table 3

Description: Genera that were found to be differentially more abundant in Never smokers vs Active smokers using DESeq2.

Abundance in Group 1: decreased abundance in Active smokers

NCBI Quality ControlLinks
Fusobacterium
Haemophilus
Methylobacterium
Streptococcus

Revision editor(s): OdigiriGreat, Folakunmi

Signature 2

Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2023/10/08

Curator: OdigiriGreat

Revision editor(s): OdigiriGreat, Folakunmi

Source: table 3

Description: Genera that were found to be differentially more abundant in Never smokers vs Active smokers using DESeq2.

Abundance in Group 1: increased abundance in Active smokers

NCBI Quality ControlLinks
Burkholderia sp.
Ralstonia sp. 1-6
Roseateles
Stenotrophomonas

Revision editor(s): OdigiriGreat, Folakunmi

Experiment 2


Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2023/10/08

Curator: OdigiriGreat

Revision editor(s): OdigiriGreat

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
former smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
subjects that are abstinent of tobacco use for at least 12months
Group 1 sample size Number of subjects in the case (exposed) group
24

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
decreased
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
decreased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
decreased

Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2023/10/09

Curator: OdigiriGreat

Revision editor(s): OdigiriGreat, Folakunmi

Source: table 3

Description: Genera that were found to be differentially more abundant in Never smokers vs former smokers using DESeq2.

Abundance in Group 1: increased abundance in former smokers

NCBI Quality ControlLinks
Ralstonia sp. 1-6

Revision editor(s): OdigiriGreat, Folakunmi

Signature 2

Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2023/10/09

Curator: OdigiriGreat

Revision editor(s): OdigiriGreat, Folakunmi

Source: table 3

Description: Genera that were found to be differentially more abundant in Never smokers vs former smokers using DESeq2.

Abundance in Group 1: increased abundance in former smokers

NCBI Quality ControlLinks
Lautropia
Leptotrichia
Prevotella sp.

Revision editor(s): OdigiriGreat, Folakunmi

Experiment 3


Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2023/10/09

Curator: OdigiriGreat

Revision editor(s): OdigiriGreat

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
former smokers
Group 1 name Corresponds to the case (exposed) group for case-control studies
active smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Participants who have smoked at least one cigarette within 3days of enrolment
Group 0 sample size Number of subjects in the control (unexposed) group
24
Group 1 sample size Number of subjects in the case (exposed) group
22

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
decreased
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
decreased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
decreased

Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2023/10/09

Curator: OdigiriGreat

Revision editor(s): OdigiriGreat, Folakunmi

Source: table 3

Description: Genera that were found to be differentially more abundant in former smokers vs Active smokers using DESeq2.

Abundance in Group 1: decreased abundance in active smokers

NCBI Quality ControlLinks
Fusobacterium
Neisseria

Revision editor(s): OdigiriGreat, Folakunmi

Signature 2

Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2023/10/09

Curator: OdigiriGreat

Revision editor(s): OdigiriGreat, Folakunmi

Source: table 3

Description: Genera that were found to be differentially more abundant in former smokers vs active smokers using DESeq2.

Abundance in Group 1: increased abundance in active smokers

NCBI Quality ControlLinks
Prevotella
Stomatobaculum

Revision editor(s): OdigiriGreat, Folakunmi

Experiment 4


Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2023/10/09

Curator: OdigiriGreat

Revision editor(s): OdigiriGreat, Folakunmi

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Active smokers & former smokers
Group 1 name Corresponds to the case (exposed) group for case-control studies
Never smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Participants who have never smoked
Group 0 sample size Number of subjects in the control (unexposed) group
46
Group 1 sample size Number of subjects in the case (exposed) group
9

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2.5

Alpha Diversity

Chao1 Abundance-based estimator of species richness
decreased
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
decreased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
decreased

Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-2-13

Curated date: 2023/10/09

Curator: OdigiriGreat

Revision editor(s): OdigiriGreat, Folakunmi

Source: Fig 5

Description: Genera that were found to be differentially more abundant in Active smokers and former smokers vs Never smokers using LEFse.

Abundance in Group 1: increased abundance in Never smokers

NCBI Quality ControlLinks
Alphaproteobacteria
Bacillota
Bacteroidales
Bacteroidia
Eubacteriaceae
Eubacterium
Haemophilus
Hyphomicrobiales
Lautropia
Megasphaera
Methylobacteriaceae
Methylobacterium
Mogibacterium
Negativicutes
Pasteurellaceae
Pasteurellales
Prevotella
Prevotellaceae
Selenomonadales
Veillonella
Veillonellaceae

Revision editor(s): OdigiriGreat, Folakunmi

Experiment 5


Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2023/10/09

Curator: OdigiriGreat

Revision editor(s): OdigiriGreat

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
never smokers & former smokers
Group 1 name Corresponds to the case (exposed) group for case-control studies
Active smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Participants who have smoked at least one cigarette within 3days of enrolment
Group 0 sample size Number of subjects in the control (unexposed) group
33
Group 1 sample size Number of subjects in the case (exposed) group
22

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
Not specified

Alpha Diversity

Chao1 Abundance-based estimator of species richness
decreased
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
decreased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
decreased

Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-2-13

Curated date: 2023/10/09

Curator: OdigiriGreat

Revision editor(s): OdigiriGreat

Source: table 3

Description: Genera that were found to be differentially more abundant in Never smokers and former smokers vs Active smokers using DESeq2.

Abundance in Group 1: decreased abundance in Active smokers

NCBI Quality ControlLinks
Delftia

Revision editor(s): OdigiriGreat

Experiment 6


Needs review

Curated date: 2024/02/16

Curator: Folakunmi

Revision editor(s): Folakunmi

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Active smokers & former smokers
Group 1 name Corresponds to the case (exposed) group for case-control studies
Never smokers
Group 0 sample size Number of subjects in the control (unexposed) group
46
Group 1 sample size Number of subjects in the case (exposed) group
9

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
increased
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
increased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
increased

Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2024/02/16

Curator: Folakunmi

Revision editor(s): Folakunmi

Source: table 3

Description: Genera that were found to be differentially more abundant in Active smokers and former smokers vs Never smokers using DESeq2.

Abundance in Group 1: increased abundance in Never smokers

NCBI Quality ControlLinks
Prevotella sp.

Revision editor(s): Folakunmi

Experiment 7


Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2024/02/16

Curator: Folakunmi

Revision editor(s): Folakunmi

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
never smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Participants who have never smoked

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
increased
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
increased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
increased

Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-2-16

Curated date: 2024/02/16

Curator: Folakunmi

Revision editor(s): Folakunmi

Source: table 3

Description: Genera that were found to be differentially more abundant in Active smokers and former smokers vs Never smokers using DESeq2.

Abundance in Group 1: increased abundance in never smokers

NCBI Quality ControlLinks
Prevotella sp.

Revision editor(s): Folakunmi