Lower respiratory tract microbiome composition and community interactions in smokers
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Lung Pulmo,Lung,lung
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Smoking status measurement Smoking status measurement,smoking status measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Never smokers
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Active smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants who have smoked at least one cigarette within 3days of enrolment
- Group 0 sample size Number of subjects in the control (unexposed) group
- 9
- Group 1 sample size Number of subjects in the case (exposed) group
- 22
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- use of antibiotics within 12weeks prior to study
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V6-V8
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Roche454
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased
Signature 1
Source: table 3
Description: Genera that were found to be differentially more abundant in Never smokers vs Active smokers using DESeq2.
Abundance in Group 1: decreased abundance in Active smokers
NCBI | Quality Control | Links |
---|---|---|
Fusobacterium | ||
Haemophilus | ||
Methylobacterium | ||
Streptococcus |
Revision editor(s): OdigiriGreat, Folakunmi
Signature 2
Source: table 3
Description: Genera that were found to be differentially more abundant in Never smokers vs Active smokers using DESeq2.
Abundance in Group 1: increased abundance in Active smokers
NCBI | Quality Control | Links |
---|---|---|
Burkholderia sp. | ||
Ralstonia sp. 1-6 | ||
Roseateles | ||
Stenotrophomonas |
Revision editor(s): OdigiriGreat, Folakunmi
Experiment 2
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- former smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- subjects that are abstinent of tobacco use for at least 12months
- Group 1 sample size Number of subjects in the case (exposed) group
- 24
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased
Signature 1
Source: table 3
Description: Genera that were found to be differentially more abundant in Never smokers vs former smokers using DESeq2.
Abundance in Group 1: increased abundance in former smokers
NCBI | Quality Control | Links |
---|---|---|
Ralstonia sp. 1-6 |
Revision editor(s): OdigiriGreat, Folakunmi
Signature 2
Source: table 3
Description: Genera that were found to be differentially more abundant in Never smokers vs former smokers using DESeq2.
Abundance in Group 1: increased abundance in former smokers
NCBI | Quality Control | Links |
---|---|---|
Lautropia | ||
Leptotrichia | ||
Prevotella sp. |
Revision editor(s): OdigiriGreat, Folakunmi
Experiment 3
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- former smokers
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- active smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants who have smoked at least one cigarette within 3days of enrolment
- Group 0 sample size Number of subjects in the control (unexposed) group
- 24
- Group 1 sample size Number of subjects in the case (exposed) group
- 22
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased
Signature 1
Source: table 3
Description: Genera that were found to be differentially more abundant in former smokers vs Active smokers using DESeq2.
Abundance in Group 1: decreased abundance in active smokers
NCBI | Quality Control | Links |
---|---|---|
Fusobacterium | ||
Neisseria |
Revision editor(s): OdigiriGreat, Folakunmi
Signature 2
Source: table 3
Description: Genera that were found to be differentially more abundant in former smokers vs active smokers using DESeq2.
Abundance in Group 1: increased abundance in active smokers
NCBI | Quality Control | Links |
---|---|---|
Prevotella | ||
Stomatobaculum |
Revision editor(s): OdigiriGreat, Folakunmi
Experiment 4
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Active smokers & former smokers
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Never smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants who have never smoked
- Group 0 sample size Number of subjects in the control (unexposed) group
- 46
- Group 1 sample size Number of subjects in the case (exposed) group
- 9
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2.5
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased
Signature 1
Source: Fig 5
Description: Genera that were found to be differentially more abundant in Active smokers and former smokers vs Never smokers using LEFse.
Abundance in Group 1: increased abundance in Never smokers
Revision editor(s): OdigiriGreat, Folakunmi
Experiment 5
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- never smokers & former smokers
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Active smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants who have smoked at least one cigarette within 3days of enrolment
- Group 0 sample size Number of subjects in the control (unexposed) group
- 33
- Group 1 sample size Number of subjects in the case (exposed) group
- 22
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- Not specified
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased
Signature 1
Source: table 3
Description: Genera that were found to be differentially more abundant in Never smokers and former smokers vs Active smokers using DESeq2.
Abundance in Group 1: decreased abundance in Active smokers
NCBI | Quality Control | Links |
---|---|---|
Delftia |
Revision editor(s): OdigiriGreat
Experiment 6
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Active smokers & former smokers
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Never smokers
- Group 0 sample size Number of subjects in the control (unexposed) group
- 46
- Group 1 sample size Number of subjects in the case (exposed) group
- 9
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- increased
- Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: table 3
Description: Genera that were found to be differentially more abundant in Active smokers and former smokers vs Never smokers using DESeq2.
Abundance in Group 1: increased abundance in Never smokers
NCBI | Quality Control | Links |
---|---|---|
Prevotella sp. |
Revision editor(s): Folakunmi
Experiment 7
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- never smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants who have never smoked
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- increased
- Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: table 3
Description: Genera that were found to be differentially more abundant in Active smokers and former smokers vs Never smokers using DESeq2.
Abundance in Group 1: increased abundance in never smokers
NCBI | Quality Control | Links |
---|---|---|
Prevotella sp. |
Revision editor(s): Folakunmi