The gut-microbiota-brain axis in a Spanish population in the aftermath of the COVID-19 pandemic: microbiota composition linked to anxiety, trauma, and depression profiles

From BugSigDB
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-20
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Malan-Müller S, Valles-Colomer M, Palomo T, Leza JC
Journal
Gut microbes
Year
2023
Keywords:
COVID-19, Microbiome, anxiety, depression, gut-microbiota-brain axis, mental health, posttraumatic stress disorder, trauma
The prevalence of anxiety and depression soared following the COVID-19 pandemic. To effectively treat these conditions, a comprehensive understanding of all etiological factors is needed. This study investigated fecal microbial features associated with mental health outcomes (symptoms of anxiety, depression, or posttraumatic stress disorder (PTSD)) in a Spanish cohort in the aftermath of the COVID-19 pandemic. Microbial communities from stool samples were profiled in 198 individuals who completed validated, self-report questionnaires. 16S ribosomal RNA gene V3-4 amplicon sequencing was performed. Microbial diversity and community structure were analyzed, together with relative taxonomic abundance. In our cohort of N=198, 17.17% reported depressive symptoms, 37.37% state anxiety symptoms, 40.90% trait anxiety symptoms, and 8.08% PTSD symptoms, with high levels of comorbidity. Individuals with trait anxiety had lower Simpson's diversity. Fusicatenibacter saccharivorans was reduced in individuals with comorbid PTSD + depression + state and trait anxiety symptoms, whilst an expansion of Proteobacteria and depletion of Synergistetes phyla were noted in individuals with depressive symptoms. The relative abundance of Anaerostipes was positively correlated with childhood trauma, and higher levels of Turicibacter sanguinis and lower levels of Lentisphaerae were found in individuals who experienced life-threatening traumas. COVID-19 infection and vaccination influenced the overall microbial composition and were associated with distinct relative taxonomic abundance profiles. These findings will help lay the foundation for future studies to identify microbial role players in symptoms of anxiety, depression, and PTSD and provide future therapeutic targets to improve mental health outcomes.

Experiment 1


Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-20

Curated date: 2023/10/29

Curator: Wangui Thuo

Revision editor(s): Wangui Thuo, Chinelsy, ChiomaBlessing

Subjects

Location of subjects
Spain
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Psychiatric disorder disease of mental health,mental disorder,mental dysfunction,mental illness,Psychiatric disease,Psychiatric disorder,psychiatric disorder
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Individuals without the comorbid state
Group 1 name Corresponds to the case (exposed) group for case-control studies
Individuals with comorbid PTSD + depression + state + trait anxiety symptoms (Comorbidity with Psychiatric Symptoms)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
This group included individuals with comorbid PTSD, depression, state and trait anxiety symptoms
Group 0 sample size Number of subjects in the control (unexposed) group
190
Group 1 sample size Number of subjects in the case (exposed) group
8
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
6 months.

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
age, body mass index, inflammatory bowel disease, sex, irritable bowel syndrome, Confounders controlled for: "celiac disease" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.celiac disease, Confounders controlled for: "Bernard-Soulier syndrome" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.Bernard-Soulier syndrome


Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-20

Curated date: 2024/02/20

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Figure 4A

Description: Relative abundance of taxa in individuals with comorbid PTSD + depression + state and trait anxiety symptoms compared to those without the comorbid state

Abundance in Group 1: decreased abundance in Individuals with comorbid PTSD + depression + state + trait anxiety symptoms (Comorbidity with Psychiatric Symptoms)

NCBI Quality ControlLinks
Fusicatenibacter saccharivorans

Revision editor(s): ChiomaBlessing

Experiment 2


Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-20

Curated date: 2024/02/20

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy controls (good mental health)
Group 1 name Corresponds to the case (exposed) group for case-control studies
PTSD + depression + state + trait anxiety symptoms (Comorbidity with Psychiatric Symptoms)
Group 0 sample size Number of subjects in the control (unexposed) group
106

Lab analysis

Statistical Analysis

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
Not specified


Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-20

Curated date: 2024/02/20

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Figure 4B

Description: Relative abundance of taxa in individuals with comorbid PTSD + depression + state and trait anxiety symptoms compared to healthy controls (good mental health)

Abundance in Group 1: decreased abundance in PTSD + depression + state + trait anxiety symptoms (Comorbidity with Psychiatric Symptoms)

NCBI Quality ControlLinks
Fusicatenibacter saccharivorans

Revision editor(s): ChiomaBlessing

Experiment 3


Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-20

Curated date: 2024/02/20

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Depressive disorder depression,melancholia,melancholias,mental depression,syndrome, depressive,syndromes, depressive,Depressive disorder,depressive disorder
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Individuals without depressive symptoms
Group 1 name Corresponds to the case (exposed) group for case-control studies
Individuals with depressive symptoms
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
This group included individuals with depressive symptoms
Group 0 sample size Number of subjects in the control (unexposed) group
166
Group 1 sample size Number of subjects in the case (exposed) group
32

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-20

Curated date: 2024/02/20

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Figure 4C

Description: Relative abundance of taxa in individuals with depressive symptoms compared to those without depressive symptoms

Abundance in Group 1: increased abundance in Individuals with depressive symptoms

NCBI Quality ControlLinks
Pseudomonadota

Revision editor(s): ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-20

Curated date: 2024/02/20

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Figure 4D

Description: Relative abundance of taxa in individuals with depressive symptoms compared to those without depressive symptoms

Abundance in Group 1: decreased abundance in Individuals with depressive symptoms

NCBI Quality ControlLinks
Synergistota

Revision editor(s): ChiomaBlessing

Experiment 4


Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-20

Curated date: 2024/02/20

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Childhood trauma measurement Childhood trauma measurement,childhood trauma measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Individuals who did not experience life-threatening childhood trauma
Group 1 name Corresponds to the case (exposed) group for case-control studies
Individuals who experienced life-threatening childhood trauma
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
This group included individuals who experienced life-threatening childhood trauma
Group 0 sample size Number of subjects in the control (unexposed) group
162
Group 1 sample size Number of subjects in the case (exposed) group
36

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-20

Curated date: 2024/02/20

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Figure 5B

Description: Relative abundance of taxa in individuals who experienced life-threatening childhood trauma compared to those who did not experience life-threatening childhood trauma

Abundance in Group 1: increased abundance in Individuals who experienced life-threatening childhood trauma

NCBI Quality ControlLinks
Turicibacter sanguinis

Revision editor(s): ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-20

Curated date: 2024/02/20

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Figure 5C

Description: Relative abundance of taxa in individuals who experienced life-threatening childhood trauma compared to those who did not experience life-threatening childhood trauma

Abundance in Group 1: decreased abundance in Individuals who experienced life-threatening childhood trauma

NCBI Quality ControlLinks
Lentisphaera

Revision editor(s): ChiomaBlessing