Gut Microbial Signatures Can Discriminate Unipolar from Bipolar Depression
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Zheng P, Yang J, Li Y, Wu J, Liang W, Yin B, Tan X, Huang Y, Chai T, Zhang H, Duan J, Zhou J, Sun Z, Chen X, Marwari S, Lai J, Huang T, Du Y, Zhang P, Perry SW, Wong ML, Licinio J, Hu S, Xie P, Wang G
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Year
2020
Keywords:
bipolar disorder, gut microbiome, major depressive disorder, microbiota–gut–brain axis, unipolar depression
Discriminating depressive episodes of bipolar disorder (BD) from major depressive disorder (MDD) is a major clinical challenge. Recently, gut microbiome alterations are implicated in these two mood disorders; however, little is known about the shared and distinct microbial characteristics in MDD versus BD. Here, using 16S ribosomal RNA (rRNA) gene sequencing, the microbial compositions of 165 subjects with MDD are compared with 217 BD, and 217 healthy controls (HCs). It is found that the microbial compositions are different between the three groups. Compared to HCs, MDD is characterized by altered covarying operational taxonomic units (OTUs) assigned to the Bacteroidaceae family, and BD shows disturbed covarying OTUs belonging to Lachnospiraceae, Prevotellaceae, and Ruminococcaceae families. Furthermore, a signature of 26 OTUs is identified that can distinguish patients with MDD from those with BD or HCs, with area under the curve (AUC) values ranging from 0.961 to 0.986 in discovery sets, and 0.702 to 0.741 in validation sets. Moreover, 4 of 26 microbial markers correlate with disease severity in MDD or BD. Together, distinct gut microbial compositions are identified in MDD compared to BD and HCs, and a novel marker panel is provided for distinguishing MDD from BD based on gut microbiome signatures.
Experiment 1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- mood disorder affective disorders,episodic mood disorder,major mood disorders,mood disorder,mood disorders
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy controls (HCs)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- bipolar disorder (BD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants who suffer from depressive episodes of bipolar disorder (BD)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 217
- Group 1 sample size Number of subjects in the case (exposed) group
- 217
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 month
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2.5
- Matched on Factors on which subjects have been matched on in a case-control study
- age, body mass index, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- decreased
- Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
- unchanged
Signature 1
Needs review Marked as Needs review by Chinelsy on 2023-11-12
Source: Supplementary file: Figure. S4b.
Description: Discriminative OTUs observed in the pairwise comparisons between bipolar disorder (BD) and healthy controls (HC).
Abundance in Group 1: increased abundance in bipolar disorder (BD)
Revision editor(s): Chinelsy, ChiomaBlessing
Experiment 2
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Major depressive disorder (MDD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants who suffer from depressive episodes from major depressive disorder (MDD)
- Group 1 sample size Number of subjects in the case (exposed) group
- 165
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
- unchanged
Signature 1
Needs review Marked as Needs review by Chinelsy on 2023-11-12
Source: Supplementary file: Figure. S4a.
Description: Discriminative OTUs observed in the pairwise comparisons between major depressive disorder (MDD) and healthy controls (HC).
Abundance in Group 1: increased abundance in Major depressive disorder (MDD)
Revision editor(s): Chinelsy, ChiomaBlessing
Experiment 3
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- bipolar disorder (BD)
Lab analysis
Statistical Analysis
- Matched on Factors on which subjects have been matched on in a case-control study
- age, body mass index, Matched on: "gender" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.gender
Signature 1
Needs review Marked as Needs review by Chinelsy on 2023-11-12
Source: Supplementary file: Figure. S4c.
Description: Discriminative OTUs observed in the pairwise comparisons between major depressive disorder (MDD) and bipolar disorder (BD).
Abundance in Group 1: increased abundance in Major depressive disorder (MDD)
Revision editor(s): Chinelsy, ChiomaBlessing
Signature 2
Needs review Marked as Needs review by Chinelsy on 2023-11-12
Source: Supplementary file: Figure. S4c.
Description: Discriminative OTUs observed in the pairwise comparisons between major depressive disorder (MDD) and bipolar disorder (BD).
Abundance in Group 1: decreased abundance in Major depressive disorder (MDD)
Revision editor(s): Chinelsy, ChiomaBlessing
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