Gut microbiome and atrial fibrillation-results from a large population-based study
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Palmu J, Börschel CS, Ortega-Alonso A, Markó L, Inouye M, Jousilahti P, Salido RA, Sanders K, Brennan C, Humphrey GC, Sanders JG, Gutmann F, Linz D, Salomaa V, Havulinna AS, Forslund SK, Knight R, Lahti L, Niiranen T, Schnabel RB
Journal
EBioMedicine
Year
2023
Keywords:
Atrial fibrillation, Epidemiology, Gut microbiome, Metagenomics
BACKGROUND: Atrial fibrillation (AF) is an important heart rhythm disorder in aging populations. The gut microbiome composition has been previously related to cardiovascular disease risk factors. Whether the gut microbial profile is also associated with the risk of AF remains unknown. METHODS: We examined the associations of prevalent and incident AF with gut microbiota in the FINRISK 2002 study, a random population sample of 6763 individuals. We replicated our findings in an independent case-control cohort of 138 individuals in Hamburg, Germany. FINDINGS: Multivariable-adjusted regression models revealed that prevalent AF (N = 116) was associated with nine microbial genera. Incident AF (N = 539) over a median follow-up of 15 years was associated with eight microbial genera with false discovery rate (FDR)-corrected P < 0.05. Both prevalent and incident AF were associated with the genera Enorma and Bifidobacterium (FDR-corrected P < 0.001). AF was not significantly associated with bacterial diversity measures. Seventy-five percent of top genera (Enorma, Paraprevotella, Odoribacter, Collinsella, Barnesiella, Alistipes) in Cox regression analyses showed a consistent direction of shifted abundance in an independent AF case-control cohort that was used for replication. INTERPRETATION: Our findings establish the basis for the use of microbiome profiles in AF risk prediction. However, extensive research is still warranted before microbiome sequencing can be used for prevention and targeted treatment of AF. FUNDING: This study was funded by European Research Council, German Ministry of Research and Education, Academy of Finland, Finnish Medical Foundation, and the Finnish Foundation for Cardiovascular Research, the Emil Aaltonen Foundation, and the Paavo Nurmi Foundation.
Experiment 1
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-13
Curated date: 2023/10/15
Curator: Iram jamshed
Revision editor(s): Iram jamshed, Chinelsy, ChiomaBlessing
Subjects
- Location of subjects
- Finland
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Atrial fibrillation A-fib,AF,AF - Atrial fibrillation,AFib,atrial fibrillation,atrial fibrillation (disease),Atrial fibrillation (disorder),Atrial Fibrillations,Auricular Fibrillation,Auricular Fibrillations,Fibrillation, Atrial,Fibrillation, Auricular,Fibrillations, Atrial,Fibrillations, Auricular,Atrial fibrillation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Individuals without Prevalent Atrial Fibrillation (AF)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Individuals with Prevalent Atrial Fibrillation (AF)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants with prevalent atrial fibrillation. Atrial fibrillation (AF) is an important heart rhythm disorder in aging populations
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6647
- Group 1 sample size Number of subjects in the case (exposed) group
- 116
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None.
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, body mass index, diabetes mellitus, physical activity, sex, smoking status, blood pressure, alcohol consumption measurement, Confounders controlled for: "heart failure" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.heart failure, Confounders controlled for: "antihypertensive medication use" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.antihypertensive medication use, total cholesterol measurement
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-13
Curated date: 2023/10/15
Curator: Iram jamshed
Revision editor(s): Iram jamshed, Tolulopeo, ChiomaBlessing
Source: Figure 2, Table 2
Description: Abundant genera in individuals with Prevalent Atrial Fibrillation (AF) compared to individuals without Prevalent AF
Abundance in Group 1: increased abundance in Individuals with Prevalent Atrial Fibrillation (AF)
| NCBI | Quality Control | Links |
|---|---|---|
| Eisenbergiella | ||
| Enorma | ||
| Enterobacter | ||
| Kluyvera |
Revision editor(s): Iram jamshed, Tolulopeo, ChiomaBlessing
Signature 2
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-13
Curated date: 2023/10/15
Curator: Iram jamshed
Revision editor(s): Iram jamshed, Tolulopeo, ChiomaBlessing
Source: Figure 2, Table 2
Description: Abundant genera in individuals with Prevalent Atrial Fibrillation (AF) compared to individuals without Prevalent AF
Abundance in Group 1: decreased abundance in Individuals with Prevalent Atrial Fibrillation (AF)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Bifidobacterium | ||
| Holdemanella | ||
| Parabacteroides | ||
| Turicibacter |
Revision editor(s): Iram jamshed, Tolulopeo, ChiomaBlessing
Experiment 2
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-13
Curated date: 2023/10/16
Curator: Iram jamshed
Revision editor(s): Iram jamshed, Tolulopeo, Chinelsy, ChiomaBlessing
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Individuals without Incident Atrial Fibrillation (AF)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Individuals with Incident Atrial Fibrillation (AF)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Incident AF refers to new cases of atrial fibrillation that occurred during the follow-up period of the study, among individuals who did not have a history of atrial fibrillation at baseline.
- Group 1 sample size Number of subjects in the case (exposed) group
- 539
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, sex, body mass index, blood pressure, smoking status, alcohol consumption measurement, diabetes mellitus, Confounders controlled for: "heart failure" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.heart failure, Confounders controlled for: "antihypertensive medication use" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.antihypertensive medication use, total cholesterol measurement
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-13
Curated date: 2023/10/17
Curator: Iram jamshed
Revision editor(s): Iram jamshed, Tolulopeo, ChiomaBlessing
Source: Figure 2, Table 3
Description: Abundant genera in individuals with Incident Atrial Fibrillation (AF) compared to individuals without Incident AF
Abundance in Group 1: increased abundance in Individuals with Incident Atrial Fibrillation (AF)
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium | ||
| Enorma | ||
| Lactococcus | ||
| Mitsuokella | ||
| Sellimonas |
Revision editor(s): Iram jamshed, Tolulopeo, ChiomaBlessing
Signature 2
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-13
Curated date: 2023/10/17
Curator: Iram jamshed
Revision editor(s): Iram jamshed, Tolulopeo, ChiomaBlessing
Source: Figure 2, Table 3
Description: Abundant genera in individuals with Incident Atrial Fibrillation (AF) compared to individuals without Incident AF
Abundance in Group 1: decreased abundance in Individuals with Incident Atrial Fibrillation (AF)
| NCBI | Quality Control | Links |
|---|---|---|
| Hungatella | ||
| Tyzzerella | ||
| Sanguibacteroides |
Revision editor(s): Iram jamshed, Tolulopeo, ChiomaBlessing
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