Feeding Rapidly Alters Microbiome Composition and Gene Transcription in the Clownfish Gut/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Chloe on 2024-4-30
Subjects
- Location of subjects
- Georgia
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Premnas biaculeatus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Digestive tract Alimentary canal,Alimentary tract,Digestive canal,Digestive tube,Enteric tract,Gut,Gut tube,Digestive tract,digestive tract
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Diet Dietary,Diets,Diet,diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Pre-fed group at 1100 h
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Post-fed group at 1230 h
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The post-fed group consists of fish fed at 1230 hours. (1.5 hours postfeeding)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 17
- Group 1 sample size Number of subjects in the case (exposed) group
- 18
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- centered log-ratio
- Statistical test
- ANCOM
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 1
Reviewed Marked as Reviewed by Chloe on 2024-4-30
Source: Supplemental Table S2
Description: The table shows the diverse microbial groups that fluctuated in abundance over the 2-day sampling period.
Abundance in Group 1: increased abundance in Post-fed group at 1230 h
Revision editor(s): Muqtadirat, Victoria
Signature 2
Reviewed Marked as Reviewed by Chloe on 2024-4-30
Source: Supplemental Table S2
Description: The table shows the diverse microbial groups that fluctuated in abundance over the 2-day sampling period.
Abundance in Group 1: decreased abundance in Post-fed group at 1230 h
| NCBI | Quality Control | Links |
|---|---|---|
| Alteromonadales |
Revision editor(s): Muqtadirat, Victoria
