Alteration of the gut microbiota profile in children with autism spectrum disorder in China

Study information

Needs review

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

38420215

DOI Digital object identifier for electronic documents.

10.3389/fmicb.2023.1326870

URI

Authors

Li H, Guo W, Li S, Sun B, Li N, Xie D, Dong Z, Luo D, Chen W, Fu W, Zheng J, Zhu J

Journal

Frontiers in microbiology

Year

2023

Keywords:

Chinese children, autism spectrum disorder, gut microbiota, gut-brain axis, metabolic pathways

BACKGROUND: Autism spectrum disorder (ASD) is associated with alterations in the gut microbiome. However, there are few studies on gut microbiota of children with ASD in China, and there is a lack of consensus on the changes of bacterial species. PURPOSE: Autism spectrum disorder (ASD) is associated with alterations in the gut microbiome. However, there are few studies on gut microbiota of children with ASD in China, and there is a lack of consensus on the changes of bacterial species. METHODS: We used 16S rRNA sequencing to analyze ASD children (2 to 12 years), HC (2 to 12 years). RESULTS: Our findings showed that the α-diversity, composition, and relative abundance of gut microbiota in the ASD group were significantly different from those in the HC groups. Compared with the HC group, the α-diversity in the ASD group was significantly decreased. At the genus level, the relative abundance of g_Faecalibacterium, g_Blautia, g_Eubacterium_eligens_group, g_Parasutterella, g_Lachnospiraceae_NK4A136_group and g_Veillonella in ASD group was significantly increased than that in HC groups, while the relative abundance of g_Prevotella 9 and g_Agathobacter was significantly decreased than that in HC groups. In addition, KEGG pathway analysis showed that the microbial functional abnormalities in ASD patients were mainly concentrated in metabolic pathways related to fatty acid, amino acid metabolism and aromatic compound metabolism, and were partially involved in neurotransmitter metabolism. CONCLUSION: This study revealed the characteristics of gut microbiota of Chinese children with ASD and provided further evidence of gut microbial dysbiosis in ASD.

Experiment 1

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Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Autism spectrum disorder atypical autism,autism spectrum disease,autism spectrum disorder,Autism Spectrum Disorders,autistic spectrum disorder,CHILD DEVELOPMENT DIS PERVASIVE,Child Development Disorders, Pervasive,Development Disorder, Pervasive,Development Disorders, Pervasive,Disorder, Autism Spectrum,Disorder, Pervasive Development,Disorders, Autism Spectrum,Disorders, Pervasive Development,PDD,PERVASIVE CHILD DEVELOPMENT DIS,Pervasive Child Development Disorders,Pervasive Development Disorder,Pervasive Development Disorders,pervasive developmental disorder - not otherwise specified,pervasive developmental disorders,Spectrum Disorder, Autism,Spectrum Disorders, Autism,Autism spectrum disorder

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy children

Group 1 name Corresponds to the case (exposed) group for case-control studies: Children with Autism Spectrum Disorder (ASD)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Diagnosed with ASD according to DSM-5 (Diagnostic and Statistical Manual of Mental Disorders - 5th Edition)

Group 0 sample size Number of subjects in the control (unexposed) group: 161

Group 1 sample size Number of subjects in the case (exposed) group: 957

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 1 month

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Chao1 Abundance-based estimator of species richness: decreased

Simpson Estimator of species richness and species evenness: more weight on species evenness: decreased

Signature 1

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Source: Figures 3A-E

Description: The shift of gut microbiota in ASD and HC groups

Abundance in Group 1: increased abundance in

NCBI	Quality Control	Links
Anaerostipes
Blautia
Lachnoclostridium
Lachnospira
Faecalibacterium
Dialister
Parasutterella
Fusobacteriia
Bacillota
Verrucomicrobiia
Veillonella
Oscillospiraceae
Lachnospiraceae
Eubacteriales
Clostridia

Revision editor(s): AlishaM, WikiWorks

Signature 2

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Curated date: 2024/10/25

Curator: AlishaM

Revision editor(s): AlishaM, WikiWorks

Source: Figures 3A-E

Description: The shift of gut microbiota in ASD and HC groups

Abundance in Group 1: decreased abundance in

NCBI	Quality Control	Links
Alistipes
Agathobacter
Subdoligranulum
Prevotellaceae
Bacteroidales
Bacteroidia
Enterobacteriaceae
Enterobacterales
Megamonas
Lentisphaerota
Methanobacteriota
Patescibacteria group

Revision editor(s): AlishaM, WikiWorks