Microbiome features associated with performance measures in athletic and non-athletic individuals: A case-control study/Experiment 6

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Reviewed Marked as Reviewed by Folakunmi on 2024-3-19

Curated date: 2024/03/08

Curator: Aleru Divine

Revision editor(s): Aleru Divine, Folakunmi

Subjects

Location of subjects
Poland
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Health study participation health questionnaire participation,health survey participation,Health study participation,health study participation
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Non-athletes
Group 1 name Corresponds to the case (exposed) group for case-control studies
Endurance athletes
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Athletes engaged in endurance athletics at timepoint W2 (morning fasting after the WT on an empty stomach)
Group 0 sample size Number of subjects in the control (unexposed) group
19
Group 1 sample size Number of subjects in the case (exposed) group
13
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
Individuals who were on antibiotics six (6) months before the start of the study.

Lab analysis

Sequencing type
PCR
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2.0
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
diet

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-3-18

Curated date: 2024/03/08

Curator: Aleru Divine

Revision editor(s): Aleru Divine, Folakunmi

Source: Table 2

Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at Timepoint W2 (morning fasting after the WT on an empty stomach)

Abundance in Group 1: increased abundance in Endurance athletes

NCBI Quality ControlLinks
Blautia sp. AF19-10LB

Revision editor(s): Aleru Divine, Folakunmi

Signature 2

Reviewed Marked as Reviewed by Folakunmi on 2024-3-18

Curated date: 2024/03/08

Curator: Aleru Divine

Revision editor(s): Aleru Divine, Folakunmi

Source: Table 2

Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at Timepoint W2 (morning fasting after the WT on an empty stomach)

Abundance in Group 1: decreased abundance in Endurance athletes

NCBI Quality ControlLinks
Phocaeicola massiliensis

Revision editor(s): Aleru Divine, Folakunmi