PandaGUT provides new insights into bacterial diversity, function, and resistome landscapes with implications for conservation/Experiment 2

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2024-4-20

Curated date: 2024/04/20

Curator: Fiddyhamma

Revision editor(s): Fiddyhamma

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Ailuropoda melanoleuca
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Diet Dietary,Diets,Diet,diet
Group 0 name Corresponds to the control (unexposed) group for case-control studies
MIN wild pandas
Group 1 name Corresponds to the case (exposed) group for case-control studies
QXL wild pandas
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Only 1864 wild giant pandas are extant and are distributed in six mountain ranges including the Qinling (QIN), Minshan (MIN), Qionglai (QIO), Daxiangling (DXL), Xiaoxiangling (XXL), and Liangshan (LSH) mountains that can be classified into three genetic populations including the QIN, MIN, and QIO-DXL-XXL-LSH (QXL) populations
Group 0 sample size Number of subjects in the control (unexposed) group
7
Group 1 sample size Number of subjects in the case (exposed) group
7

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased