Differential peripheral immune signatures elicited by vegan versus ketogenic diets in humans/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-7-30
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Diet Dietary,Diets,Diet,diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Participants on vegan diet
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Participants on ketogenic diet
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- To determine the impact of dietary intervention on human immunity and microbiota, this group of participants were placed on a ketogenic diet, and would later be switched to a vegan diet.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 10
- Group 1 sample size Number of subjects in the case (exposed) group
- 10
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- centered log-ratio
- Statistical test
- MaAsLin2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-7-30
Source: Fig. 4e
Description: Differentially abundant species between ketogenic and vegan diets for all significant taxa, showing the impact of a ketogenic diet on participants compared to a vegan diet.
Abundance in Group 1: increased abundance in Participants on ketogenic diet
Revision editor(s): Johnpaul, Aleru Divine, Scholastica
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-7-30
Source: Fig. 4e
Description: Differentially abundant species between ketogenic and vegan diets for all significant taxa, showing the impact of a ketogenic diet on participants compared to a vegan diet.
Abundance in Group 1: decreased abundance in Participants on ketogenic diet
Revision editor(s): Johnpaul, Aleru Divine, Scholastica
