Influence of aerobic exercise training on mice gut microbiota in Parkinson's disease

Study information

Reviewed Marked as Reviewed by Folakunmi on 2024-4-1

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

37529095

DOI Digital object identifier for electronic documents.

10.55730/1300-0152.2617

URI

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388120/

Authors

Fan T, Li X, Zhang X, Zhang J, Sun L, Chen J, Fu C

Journal

Turkish journal of biology = Turk biyoloji dergisi

Year

2022

Keywords:

16S rRNA gene sequencing, Parkinson’s disease, aerobic exercise training, gut microbiota

Accumulating evidence shows that gut microbial dysbiosis may represent a risk factor for Parkinson's disease (PD). Exercise has a positive effect on microbiota in general. The effect of aerobic exercise training (AET) on the gut microbial environment in PD remains to be explored. Here, we performed the 16S rRNA gene sequencing on feces from sham operated-mice (sham), PD mice model, and mice receiving AET (AET). Results indicated that AET had no remarkable effect on species richness and bacterial diversity of PD mice. The relative abundance of the Bacteroidetes was reduced, while Firmicutes, Actinobacteria, Lactobacillaceae, Streptococcaceae, Lactobacillus, Streptococcus, Lactococcus, Lysinibacillus, Pelomonas, and Prevotellaceae_UCG-001 were increased in PD mice compared with those of sham operated-mice, whereas AET partly rescued their abundance. Additionally, the composition proportion of beneficial Lactobacillus_gasseri and uncultured_Erysipelotrichales_bacterium significantly increased in AET mice compared to PD mice. Moreover, discriminative bacteria, such as Bacilli, Lactobacillales, Lactobacillaceae, Lactobacillus, and Lactococcus were identified as a specific taxon in AET mice. Here we provide evidence that AET can improve the gut microbiota of PD mice.

Experiment 1

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Reviewed Marked as Reviewed by Folakunmi on 2024-4-1

Curated date: 2024/03/09

Curator: EGO

Revision editor(s): EGO, Folakunmi

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Response to exercise Response to exercise,response to exercise

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Sham group and parkison's disease (PD) group

Group 1 name Corresponds to the case (exposed) group for case-control studies: Aerobic exercise training group (AET)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Mice group that received grasping, rotating, walking and balance training exercises once a day, 6 days a week, 30 min each time, a total of 4 weeks.

Group 0 sample size Number of subjects in the control (unexposed) group: 10

Group 1 sample size Number of subjects in the case (exposed) group: 5

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 4

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Folakunmi on 2024-4-1

Curated date: 2024/03/09

Curator: EGO

Revision editor(s): EGO, Welile, Folakunmi

Source: Figure 6

Description: Discriminative taxon in sham, PD, and AET groups by linear discriminant analysis (LDA) integrated with effect size (LEfSe)

Abundance in Group 1: increased abundance in Aerobic exercise training group (AET)

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Bacilli
Lactobacillales
Lactobacillaceae
Lactobacillus
Lactococcus
Lysinibacillus
Roseateles
Caryophanaceae
Faecalibacterium
Rhodobacter
uncultured Prevotella sp.

Revision editor(s): EGO, Welile, Folakunmi

Experiment 2

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Curated date: 2024/04/01

Curator: Folakunmi

Revision editor(s): Folakunmi

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Sham group and Aerobic exercise training group (AET) group

Group 1 name Corresponds to the case (exposed) group for case-control studies: parkison's disease (PD) group

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Mice group that were administered intraperitoneally with 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) at 30 mg/kg for 7 days to construct PD mouse models.

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Signature 1

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Source: Figure 6

Description: Discriminative taxon in sham, PD, and AET groups by linear discriminant analysis (LDA) integrated with effect size (LEfSe)

Abundance in Group 1: increased abundance in parkison's disease (PD) group

NCBI	Quality Control	Links
Dubosiella
Thermomonosporaceae
Ileibacterium
Deltaproteobacteria
Enterorhabdus

Revision editor(s): Folakunmi

Experiment 3

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Reviewed Marked as Reviewed by Folakunmi on 2024-4-1

Curated date: 2024/04/01

Curator: Folakunmi

Revision editor(s): Folakunmi

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Aerobic exercise training group (AET) and parkison's disease (PD) group

Group 1 name Corresponds to the case (exposed) group for case-control studies: Sham group

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: group of mice that were injected intraperitoneally with equivalent volumes of normal saline for 7 days and did not receive any training