Differential Analysis of Gut Microbiota Correlated With Oxidative Stress in Sows With High or Low Litter Performance During Lactation/Experiment 2

From BugSigDB


Needs review

Curated date: 2024/04/14

Curator: Blessingpeters

Revision editor(s): Blessingpeters, Scholastica

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Sus scrofa domesticus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Gut microbiome measurement Gut microbiome measurement,gut microbiome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
High litter performance sows at L21
Group 1 name Corresponds to the case (exposed) group for case-control studies
Low litter performance sows at L21
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Low litter performance sows group at day 21 of lactation (L21)
Group 0 sample size Number of subjects in the control (unexposed) group
10
Group 1 sample size Number of subjects in the case (exposed) group
10
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
None

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
decreased
Richness Number of species
unchanged

Signature 1

Needs review

Curated date: 2024/04/15

Curator: Scholastica

Revision editor(s): Scholastica

Source: Figure 4E

Description: Differences in fecal microbiota between day 21 of lactation of high litter performance sows compared to day 21 of lactation of low litter performance sows

Abundance in Group 1: increased abundance in Low litter performance sows at L21

NCBI Quality ControlLinks
Clostridia
Eubacteriales

Revision editor(s): Scholastica

Signature 2

Needs review

Curated date: 2024/04/15

Curator: Scholastica

Revision editor(s): Scholastica

Source: Figure 4E

Description: Differences in fecal microbiota between day 21 of lactation of high litter performance sows compared to day 21 of lactation of low litter performance sows

Abundance in Group 1: decreased abundance in Low litter performance sows at L21

NCBI Quality ControlLinks
unclassified Mollicutes
Parabacteroides
Porphyromonadaceae
Mycoplasmatota
Mollicutes
Coprococcus
o_RF39o_RF39
f_norank_o_RF39f_norank_o_RF39
g_norank_o_RF39g_norank_o_RF39

Revision editor(s): Scholastica