Differential Analysis of Gut Microbiota Correlated With Oxidative Stress in Sows With High or Low Litter Performance During Lactation/Experiment 3

From BugSigDB


Needs review

Curated date: 2024/04/14

Curator: Blessingpeters

Revision editor(s): Blessingpeters, Scholastica

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Sus scrofa domesticus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Gut microbiome measurement Gut microbiome measurement,gut microbiome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Day 109 of gestation (G109) of H group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Day 21 of lactation (L21) of H group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Day 21 of lactation (L21) of high litter performance sows group
Group 0 sample size Number of subjects in the control (unexposed) group
10
Group 1 sample size Number of subjects in the case (exposed) group
10
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
None

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
decreased

Signature 1

Needs review

Curated date: 2024/04/14

Curator: Scholastica

Revision editor(s): Scholastica

Source: Figure 4H

Description: Differences in fecal microbiota between day 109 of gestation (G109) compared to day 21 of lactation (L21) of high litter performance sows

Abundance in Group 1: increased abundance in Day 21 of lactation (L21) of H group

NCBI Quality ControlLinks
Lactobacillaceae
Mollicutes
Mycoplasmatota
Parabacteroides
Porphyromonadaceae
Ruminococcus
unclassified Mogibacterium
RF39RF39
f_norank_o_RF39f_norank_o_RF39
g_norank_o_RF39g_norank_o_RF39
g_norank_f_WCHB1_25g_norank_f_WCHB1_25
f_WCHB1_25f_WCHB1_25
g_norank_f_Leuconostocaceaeg_norank_f_Leuconostocaceae
CF231CF231

Revision editor(s): Scholastica

Signature 2

Needs review

Curated date: 2024/04/14

Curator: Scholastica

Revision editor(s): Scholastica

Source: Figure 4H

Description: Differences in fecal microbiota between day 109 of gestation (G109) compared to day 21 of lactation (L21) of high litter performance sows

Abundance in Group 1: decreased abundance in Day 21 of lactation (L21) of H group

NCBI Quality ControlLinks
Bacteroides
Bacteroidaceae
Pseudomonadota
Gammaproteobacteria
Nocardioidaceae
Propionicimonas
Succinivibrio
Aeromonadales
Succinivibrionaceae
unclassified Coriobacteriaceae
unclassified Helicobacteraceae
f_norank_c_TM7_3f_norank_c_TM7_3
o_norank_c_TM7_3o_norank_c_TM7_3
g_norank_c_TM7_3g_norank_c_TM7_3
g_L7A_E11g_L7A_E11

Revision editor(s): Scholastica