Background:
Infantile cholestasis (IC) is the most common hepatobiliary disease in infants, resulting in elevated direct bilirubin levels. Indeed, hepatointestinal circulation impacts bile acid and bilirubin metabolism. This study evaluates changes in the gut microbiota composition in children with IC and identifies abnormal metabolite profiles associated with microbial alterations.
Results:
The gut microbiota in the IC group exhibits a higher abundance of Veillonella, Streptococcus and Clostridium spp. (P<0.05), compared to the healthy infants (CON) group. Moreover, the abundance of Ruminococcus, Vibrio butyricum, Eubacterium coprostanogenes group, Intestinibacter, and Faecalibacterium were lower (P<0.05). In terms of microbiota-derived metabolites, the levels of fatty acids (palmitoleic, α-linolenic, arachidonic, and linoleic) (P<0.05) increased and the levels of amino acids decreased in IC group. Furthermore, the abundance of Ruminococcus, Eubacterium coprostanoligenes group, Intestinibacter and Butyrivibrio are positively correlated with proline, asparagine and aspartic acid, but negatively correlated with the α-linolenic acid, linoleic acid, palmitoleic acid and arachidonic acid. For analysis of the relationship between the microbiota and clinical index, it was found that the abundance of Veillonella and Streptococcus was positively correlated with serum bile acid content (P<0.05), while APTT, PT and INR were negatively correlated with Faecalibalum and Ruminococcus (P<0.05).
Conclusion:
Microbiota dysbiosis happens in IC children, which also can lead to abnormal metabolism,
thus obstructing the absorption of enteral nutrition and aggravating liver cell damage. Veillonella, Ruminococcus and Butyrivibrio may be important microbiome related to IC and need further research.
Keywords:
Infantile cholestasis (IC), Gut microbiota, Microbiota-derived metabolites, Ruminococcus, Butyrivibrio,
Veillonella
Experiment 1
Reviewed Marked as Reviewed by Peace Sandy on 2024-4-3
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy Control (CON)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Infantile cholestasis (IC)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Infants aged less than 1 year with all diagnostic criteria for cholestasis met upon admission.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 20
- Group 1 sample size Number of subjects in the case (exposed) group
- 20
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- No antibiotics were administered over the preceding 2 weeks.
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- Matched on Factors on which subjects have been matched on in a case-control study
- age
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Peace Sandy on 2024-4-3
Source: Figure 1 C
Description: Characteristics of gut microbiota in infantile cholestasis (IC; n = 20) and control (CON; n = 20) groups. C. score plot of differential species showing linear discriminant analysis of differentially abundant genera between the two groups;
Abundance in Group 1: increased abundance
in
Infantile cholestasis (IC)
| NCBI | Quality Control | Links |
|---|
| Dysgonomonadaceae | | |
| Dysgonomonas | | |
| Gammaproteobacteria | | |
| Lachnoclostridium | | |
| Pseudomonadota | | |
| Streptococcaceae | | |
| Streptococcus | | |
Revision editor(s): Amara,
Ayibatari,
Peace Sandy
Signature 2
Reviewed Marked as Reviewed by Peace Sandy on 2024-4-3
Source: Figure 1 C
Description: Characteristics of gut microbiota in infantile cholestasis (IC; n = 20) and control (CON; n = 20) groups. C. score plot of differential species showing linear discriminant analysis of differentially abundant genera between the two groups;
Abundance in Group 1: decreased abundance
in
Infantile cholestasis (IC)
| NCBI | Quality Control | Links |
|---|
| Clostridia | | |
| Oscillospiraceae | | |
| Romboutsia | | |
| Erysipelotrichaceae | | |
| Erysipelotrichales | | |
| Caulobacter | | |
| Thomasclavelia | | |
| Christensenellaceae | | |
| Christensenellales | | |
| Amorphus | | |
| Rhodobiaceae | | |
Revision editor(s): Amara,
Welile,
Peace Sandy
