Curcumin Regulates Gut Microbiota and Exerts a Neuroprotective Effect in the MPTP Model of Parkinson's Disease
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Zhu H, Zhang H, Hou B, Xu B, Ji L, Wu Y
Journal
Evidence-based complementary and alternative medicine : eCAM
Year
2022
OBJECTIVES: The experiment aimed to explore the effects of curcumin on motor impairment, dopamine neurons, and gut microbiota in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mice model. METHODS: Mice were randomly assigned to six groups: normal control group, solvent control group, MPTP group, curcumin-low-dose group (40 mg/kg), curcumin-medium-dose group (80 mg/kg), and curcumin-high-dose group (160 mg/kg). After 14 days, each group of mice was subjected to the pole text, the hanging test, and the open-field test. Tyrosine hydroxylase (TH) immunohistochemistry was used to observe the survival of nigrostriatal dopamine neurons. Moreover, ultrastructural changes were observed with a transmission electron microscope in mice striatal tissue cells. Then, 16S rRNA was used to assess changes in the gut microbiota. RESULTS: (1) Each dose of curcumin reduced pole climbing time and increased suspension score and total distance moved dose-dependently. (2) All curcumin groups improved cell wrinkling and vacuolar degeneration, increased the number of TH positives, improved cell survival, and the higher the dose of curcumin, the better the effect. (3) There were differences in microbiota composition and a relative abundance among the groups. The relative abundance of Patescibacteria, Proteobacteria, and Verrucomicrobia was higher in the MPTP group. The relative abundance of Patescibacteria, Enterobacteriaceae, Enterococcaceae all decreased in all curcumin groups. In addition, the Kyoto Encyclopedia of Genes and Genomes pathways showed a reduction in the superpathway of N-acetylneuraminate degradation after medium- and high-dose curcumin administration. CONCLUSIONS: Curcumin regulates gut microbiota and exerts a neuroprotective effect in the MPTP mice model. This preliminary study demonstrates the therapeutic potential of curcumin for Parkinson's disease, providing clues for microbially targeted therapies for Parkinson's disease.
Experiment 1
Reviewed Marked as Reviewed by Svetlana up on 2024-7-1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Curcumin (1E,6E)-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,6-diene-3,5-dione,C21,C21H20O6,COc1cc(ccc1O)\\\\C=C\\\\C(=O)CC(=O)\\\\C=C\\\\c1ccc(O)c(OC)c1,DC42,Diferuloylmethane,FLJ12894,InChI=1/C21H20O6/c1-26-20-11-14(5-9-18(20)24)3-7-16(22)13-17(23)8-4-15-6-10-19(25)21(12-15)27-2/h3-12,24-25H,13H2,1-2H3/b7-3+,8-4+,InChIKey=VFLDPWHFBUODDF-FCXRPNKRBF,IRA1,Kacha haldi,Natural yellow 3,TBL1XR1 wt Allele,TBLR1,Transducin (Beta)-Like 1 X-Linked Receptor 1 wt Allele,Transducin (Beta)-Like 1X-Linked Receptor 1 Gene,Turmeric yellow,Curcumin,curcumin
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- MPTP
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CUR-L
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Curcumin low-dose (CUR-L) group received a combination of both treatments in which curcumin (40 mg/kg)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 12
- Group 1 sample size Number of subjects in the case (exposed) group
- 12
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 3
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-7-1
Source: Fig 5 and Text
Description: Differential distribution of Specie in the gut Microbiota between MPTP and CUR-L.
Abundance in Group 1: increased abundance in CUR-L
Revision editor(s): MyleeeA
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-7-1
Source: Fig 5 and Text
Description: Differential distribution of Specie in the gut Microbiota between MPTP and CUR-L .
Abundance in Group 1: decreased abundance in CUR-L
Revision editor(s): MyleeeA
Experiment 2
Reviewed Marked as Reviewed by Svetlana up on 2024-7-1
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CUR-M
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Curcumin Medium-dose (CUR-M) group received a combination of both treatments in which curcumin (80 mg/kg)
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-7-1
Source: Fig 5 and Text
Description: Differential distribution of Specie in the gut Microbiota between MPTP and CUR-M.
Abundance in Group 1: increased abundance in CUR-M
Revision editor(s): MyleeeA
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-7-1
Source: Fig 5 and Text
Description: Differential distribution of Specie in the gut Microbiota between MPTP and CUR-M.
Abundance in Group 1: decreased abundance in CUR-M
Revision editor(s): MyleeeA
Experiment 3
Reviewed Marked as Reviewed by Svetlana up on 2024-7-1
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CUR-H
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Curcumin High-dose (CUR-H) group received a combination of both treatments in which curcumin (160 mg/kg)
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-7-1
Source: Fig 5 and Text
Description: Differential distribution of Specie in the gut Microbiota between MPTP and CUR-H.
Abundance in Group 1: increased abundance in CUR-H
Revision editor(s): MyleeeA
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-7-1
Source: Fig 5 and Text
Description: Differential distribution of Specie in the gut Microbiota between MPTP and CUR-H.
Abundance in Group 1: decreased abundance in CUR-H
Revision editor(s): MyleeeA
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