Curcumin Regulates Gut Microbiota and Exerts a Neuroprotective Effect in the MPTP Model of Parkinson's Disease

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Reviewed Marked as Reviewed by Svetlana up on 2024-7-1
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Zhu H, Zhang H, Hou B, Xu B, Ji L, Wu Y
Journal
Evidence-based complementary and alternative medicine : eCAM
Year
2022
OBJECTIVES: The experiment aimed to explore the effects of curcumin on motor impairment, dopamine neurons, and gut microbiota in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mice model. METHODS: Mice were randomly assigned to six groups: normal control group, solvent control group, MPTP group, curcumin-low-dose group (40 mg/kg), curcumin-medium-dose group (80 mg/kg), and curcumin-high-dose group (160 mg/kg). After 14 days, each group of mice was subjected to the pole text, the hanging test, and the open-field test. Tyrosine hydroxylase (TH) immunohistochemistry was used to observe the survival of nigrostriatal dopamine neurons. Moreover, ultrastructural changes were observed with a transmission electron microscope in mice striatal tissue cells. Then, 16S rRNA was used to assess changes in the gut microbiota. RESULTS: (1) Each dose of curcumin reduced pole climbing time and increased suspension score and total distance moved dose-dependently. (2) All curcumin groups improved cell wrinkling and vacuolar degeneration, increased the number of TH positives, improved cell survival, and the higher the dose of curcumin, the better the effect. (3) There were differences in microbiota composition and a relative abundance among the groups. The relative abundance of Patescibacteria, Proteobacteria, and Verrucomicrobia was higher in the MPTP group. The relative abundance of Patescibacteria, Enterobacteriaceae, Enterococcaceae all decreased in all curcumin groups. In addition, the Kyoto Encyclopedia of Genes and Genomes pathways showed a reduction in the superpathway of N-acetylneuraminate degradation after medium- and high-dose curcumin administration. CONCLUSIONS: Curcumin regulates gut microbiota and exerts a neuroprotective effect in the MPTP mice model. This preliminary study demonstrates the therapeutic potential of curcumin for Parkinson's disease, providing clues for microbially targeted therapies for Parkinson's disease.

Experiment 1


Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/12

Curator: MyleeeA

Revision editor(s): MyleeeA

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Curcumin (1E,6E)-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,6-diene-3,5-dione,C21,C21H20O6,COc1cc(ccc1O)\\\\C=C\\\\C(=O)CC(=O)\\\\C=C\\\\c1ccc(O)c(OC)c1,DC42,Diferuloylmethane,FLJ12894,InChI=1/C21H20O6/c1-26-20-11-14(5-9-18(20)24)3-7-16(22)13-17(23)8-4-15-6-10-19(25)21(12-15)27-2/h3-12,24-25H,13H2,1-2H3/b7-3+,8-4+,InChIKey=VFLDPWHFBUODDF-FCXRPNKRBF,IRA1,Kacha haldi,Natural yellow 3,TBL1XR1 wt Allele,TBLR1,Transducin (Beta)-Like 1 X-Linked Receptor 1 wt Allele,Transducin (Beta)-Like 1X-Linked Receptor 1 Gene,Turmeric yellow,Curcumin,curcumin
Group 0 name Corresponds to the control (unexposed) group for case-control studies
MPTP
Group 1 name Corresponds to the case (exposed) group for case-control studies
CUR-L
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Curcumin low-dose (CUR-L) group received a combination of both treatments in which curcumin (40 mg/kg)
Group 0 sample size Number of subjects in the control (unexposed) group
12
Group 1 sample size Number of subjects in the case (exposed) group
12

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/13

Curator: MyleeeA

Revision editor(s): MyleeeA

Source: Fig 5 and Text

Description: Differential distribution of Specie in the gut Microbiota between MPTP and CUR-L.

Abundance in Group 1: increased abundance in CUR-L

NCBI Quality ControlLinks
Adlercreutzia
Adlercreutzia equolifaciens
Alistipes
Alistipes inops
Alistipes sp. S457
Alloprevotella
Alloprevotella tannerae
Anaeroplasma
Anaeroplasmataceae
Anaeroplasmatales
Anaerotignum
Anaerotignum sp.
Bacteroidales
Bacteroidia
Candidatus Gastranaerophilales
Cyanobacteriota
Cyanobacteriota/Melainabacteria group
Eggerthellaceae
Eggerthellales
Lachnoclostridium
Lachnoclostridium sp.
Lactobacillus sp. L-YJ
Paramuribaculum
Paramuribaculum intestinale
Prevotellaceae
Rikenellaceae
[Eubacterium] brachy
unclassified Anaeroplasma
unclassified Candidatus Gastranaerophilales
unclassified sequences
uncultured Clostridium sp.
Eubacterium brachy group unclassifedEubacterium brachy group unclassifed

Revision editor(s): MyleeeA

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/14

Curator: MyleeeA

Revision editor(s): MyleeeA

Source: Fig 5 and Text

Description: Differential distribution of Specie in the gut Microbiota between MPTP and CUR-L .

Abundance in Group 1: decreased abundance in CUR-L

NCBI Quality ControlLinks
Akkermansia
Akkermansia muciniphila
Akkermansiaceae
Atopobiaceae
Bacteria
Candidatus Saccharimonadaceae
Candidatus Saccharimonadales
Candidatus Saccharimonadia
Candidatus Saccharimonas
Eubacteriaceae
Eubacterium
Intestinimonas
Lentilactobacillus hilgardii
Limosilactobacillus reuteri
Muribaculum intestinale
Patescibacteria group
Verrucomicrobiales
Verrucomicrobiia
Verrucomicrobiota
unclassified Candidatus Saccharimonas
unclassified Intestinimonas
uncultured Bifidobacterium sp.
Coriobacteriaceae_UCG_002Coriobacteriaceae_UCG_002
Coriobacteriaceae_UCG_002_unclassifedCoriobacteriaceae_UCG_002_unclassifed

Revision editor(s): MyleeeA

Experiment 2


Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/14

Curator: MyleeeA

Revision editor(s): MyleeeA

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
CUR-M
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Curcumin Medium-dose (CUR-M) group received a combination of both treatments in which curcumin (80 mg/kg)

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/14

Curator: MyleeeA

Revision editor(s): MyleeeA

Source: Fig 5 and Text

Description: Differential distribution of Specie in the gut Microbiota between MPTP and CUR-M.

Abundance in Group 1: increased abundance in CUR-M

NCBI Quality ControlLinks
Bacteroides acidifaciens
Betaproteobacteria
Burkholderiaceae
Eggerthellaceae
Enterorhabdus
Eubacterium coprostanoligenes
Lachnoclostridium sp.
Paramuribaculum
Paramuribaculum intestinale
Prevotellaceae
Pseudomonadaceae
Pseudomonadales
Pseudomonas
Pseudomonas sp. DENB20
Rikenellaceae
unclassified Enterorhabdus
uncultured Streptococcus sp.
Ruminococcaceae UCG 010 unclassifedRuminococcaceae UCG 010 unclassifed
Eubacterium coprostanoligenes group unclassifedEubacterium coprostanoligenes group unclassifed
Ruminococcaceae UCG 010Ruminococcaceae UCG 010

Revision editor(s): MyleeeA

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/14

Curator: MyleeeA

Revision editor(s): MyleeeA

Source: Fig 5 and Text

Description: Differential distribution of Specie in the gut Microbiota between MPTP and CUR-M.

Abundance in Group 1: decreased abundance in CUR-M

NCBI Quality ControlLinks
Acetatifactor
Acetatifactor sp.
Eubacteriaceae
Eubacterium
Lentilactobacillus hilgardii
Limosilactobacillus reuteri
uncultured Eubacterium sp.
Coriobacteriaceae UCG 002 unclassifedCoriobacteriaceae UCG 002 unclassifed
Coriobacteriaceae UCG 002Coriobacteriaceae UCG 002

Revision editor(s): MyleeeA

Experiment 3


Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/14

Curator: MyleeeA

Revision editor(s): MyleeeA

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
CUR-H
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Curcumin High-dose (CUR-H) group received a combination of both treatments in which curcumin (160 mg/kg)

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/14

Curator: MyleeeA

Revision editor(s): MyleeeA

Source: Fig 5 and Text

Description: Differential distribution of Specie in the gut Microbiota between MPTP and CUR-H.

Abundance in Group 1: increased abundance in CUR-H

NCBI Quality ControlLinks
Actinomycetota
Allobaculum
Bacillota
Coriobacteriales
Coriobacteriia
Eggerthellaceae
Enterorhabdus
Intestinimonas
Lactobacillus sp. L-YJ
Mollicutes
Mycoplasmatota
Paramuribaculum
Paramuribaculum intestinale
Pseudomonadaceae
Pseudomonadales
Pseudomonas
Pseudomonas sp. DENB20
unclassified Enterorhabdus
unclassified Intestinimonas
uncultured Allobaculum sp.
uncultured Ruminiclostridium sp.
Ruminiclostridium 9Ruminiclostridium 9

Revision editor(s): MyleeeA

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/15

Curator: MyleeeA

Revision editor(s): MyleeeA

Source: Fig 5 and Text

Description: Differential distribution of Specie in the gut Microbiota between MPTP and CUR-H.

Abundance in Group 1: decreased abundance in CUR-H

NCBI Quality ControlLinks
Candidatus Saccharimonadales
Candidatus Saccharimonadia
Eubacteriaceae
Eubacterium
Eubacterium coprostanoligenes
Holdemania
Holdemania massiliensis
Lentilactobacillus hilgardii
Limosilactobacillus reuteri
Patescibacteria group
[Eubacterium] nodatum
unclassified Candidatus Saccharimonas
uncultured Bacteroides sp.
uncultured Eubacterium sp.
Eubacterium nodatum group unclassifedEubacterium nodatum group unclassifed
Eubacterium coprostanoligenes groupEubacterium coprostanoligenes group
Family XIIIFamily XIII
Candidatus Saccharimonas

Revision editor(s): MyleeeA