Exploring the alteration of gut microbiota and brain function in gender-specific Parkinson's disease based on metagenomic sequencing

Study information

Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

37502423

DOI Digital object identifier for electronic documents.

10.3389/fnagi.2023.1148546

URI

Authors

Zhang M, Zhai Z, Yang B, He L, Wang J, Dai W, Xue L, Yang X, Feng Y, Wang H

Journal

Frontiers in aging neuroscience

Year

2023

Keywords:

Parkinson’s disease, fMRI, gender, gut microbiota, metagenomic sequencing

BACKGROUND: The role of the microbiota-gut-brain axis in Parkinson's disease (PD) has received increasing attention. Although gender differences are known to an essential role in the epidemiology and clinical course of PD, there are no studies on the sex specificity of the microbiota-gut-brain axis in the development and progression of PD. METHODS: Fresh fecal samples from 24 PD patients (13 males, 11 females) were collected for metagenomic sequencing. The composition and function of the gut microbiota were analyzed by resting-state functional magnetic resonance imaging (fMRI). Gender-dependent differences in brain ALFF values and their correlation with microbiota were further analyzed. RESULTS: The relative abundance of Propionivibrio, Thermosediminibacter, and Flavobacteriaceae_noname was increased in male PD patients. LEfse analysis showed that Verrucomicrobial, Akkermansiaceae, and Akkermansia were dominant in the males. In female patients, the relative abundance of Propionicicella was decreased and Escherichia, Escherichia_coli, and Lachnospiraceae were predominant. The expression of the sesquiterpenoid and triterpenoid biosynthesis pathways was increased in male PD patients and was statistically different from females. Compared to the Male PD patients, female patients showed decreased ALFF values in the left inferior parietal regions, and the relative abundance of Propionivibrio was positively correlated with the regional ALFF values. CONCLUSION: Our study provides novel clinical evidence of the gender-specific relationship between gut microbiota alterations and brain function in PD patients, highlighting the critical role of the microbiota-gut-brain axis in gender differences in PD.

Experiment 1

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Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/03/14

Curator: Keamy

Revision editor(s): Keamy, Scholastica

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Parkinson's disease IDIOPATHIC PARKINSON DIS,Idiopathic Parkinson Disease,Idiopathic Parkinson's Disease,IDIOPATHIC PARKINSONS DIS,Idiopathic PD,LEWY BODY PARKINSON DIS,Lewy Body Parkinson Disease,Lewy Body Parkinson's Disease,Paralysis agitans,paralysis agitans,PARKINSON DIS,PARKINSON DIS IDIOPATHIC,Parkinson disease,Parkinson Disease, Idiopathic,Parkinson syndrome,Parkinson's,Parkinson's disease,Parkinson's disease (disorder),Parkinson's disease NOS,Parkinson's disease NOS (disorder),Parkinson's Disease, Idiopathic,Parkinson's Disease, Lewy Body,Parkinson's syndrome,Parkinsonian disorder,Parkinsonism, Primary,Parkinsons,PARKINSONS DIS,PARKINSONS DIS IDIOPATHIC,PARKINSONS DIS LEWY BODY,Parkinsons disease,Primary Parkinsonism,parkinson's disease

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Female Parkinson’s disease (PD_F) patients

Group 1 name Corresponds to the case (exposed) group for case-control studies: Male Parkinson’s disease (PD_M) patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Male patients with Parkinson’s disease (PD) diagnosed according to the primary Parkinson’s disease diagnostic criteria.

Group 0 sample size Number of subjects in the control (unexposed) group: 11

Group 1 sample size Number of subjects in the case (exposed) group: 13

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): History of antibiotic use in the last month

Lab analysis

Sequencing type: WMS

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 3.0

Matched on Factors on which subjects have been matched on in a case-control study: age, body weight, Matched on: "Hoehn-Yahr (HY) score" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.Hoehn-Yahr (HY) score

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

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Curated date: 2024/03/14

Curator: Keamy

Revision editor(s): Keamy, Scholastica

Source: Fig. 2C, 2E

Description: Significantly differential taxa identified between male and female Parkinson’s disease (PD) patients

Abundance in Group 1: increased abundance in Male Parkinson’s disease (PD_M) patients

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Akkermansia
Akkermansia muciniphila
Akkermansiaceae
Alsobacter
Candidatus Soleaferrea
Dethiosulfatibacter
Halocella
Leminorella
Propionivibrio
Thermosediminibacter
Verrucomicrobiales
Verrucomicrobiia
Verrucomicrobiota
unclassified Bacteria
unclassified Flavobacteriaceae

Revision editor(s): Keamy, Scholastica

Signature 2

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Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/03/14

Curator: Keamy

Revision editor(s): Keamy, Scholastica

Source: Fig. 2C, 2E

Description: Significantly differential taxa identified between male and female Parkinson’s disease (PD) patients

Abundance in Group 1: decreased abundance in Male Parkinson’s disease (PD_M) patients

NCBI	Quality Control	Links
Agathobacter rectalis
Coprococcus
Escherichia
Escherichia coli
Lachnospiraceae
Propionicicella
Roseburia intestinalis
Ruminococcus
Ruminococcus sp. CAG:254
[Ruminococcus] torques
unclassified Escherichia
unclassified Lachnospiraceae
unclassified Ruminococcus

Revision editor(s): Keamy, Scholastica