Gut Microbiome Alterations following Postnatal Iron Supplementation Depend on Iron Form and Persist into Adulthood

From BugSigDB
Needs review
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
McMillen S, Thomas S, Liang E, Nonnecke EB, Slupsky C, Lönnerdal B
Journal
Nutrients
Year
2022
Keywords:
ferrous bis-glycinate chelate, ferrous sulfate, gut microbiome, infant nutrition, iron supplement, metabolomics, rat model
The gut microbiota is implicated in the adverse developmental outcomes of postnatal iron supplementation. To generate hypotheses on how changes to the gut microbiota by iron adversely affect development, and to determine whether the form of iron influences microbiota outcomes, we characterized gut microbiome and metabolome changes in Sprague-Dawley rat pups given oral supplements of ferrous sulfate (FS), ferrous bis-glycinate chelate (FC), or vehicle control (CON) on postnatal day (PD) 2−14. Iron supplementation reduced microbiome alpha-diversity (p < 0.0001) and altered short-chain fatty acids (SCFAs) and trimethylamine (TMA) in a form-dependent manner. To investigate the long-term effects of iron provision in early life, an additional cohort was supplemented with FS, FC, or CON until PD 21 and then weaned onto standard chow. At ~8 weeks of age, young adult (YA) rats that received FS exhibited more diverse microbiomes compared to CON (p < 0.05), whereas FC microbiomes were less diverse (p < 0.05). Iron provision resulted in 10,000-fold reduced abundance of Lactobacilli in pre-weanling and YA animals provided iron in early life (p < 0.0001). Our results suggest that in pre-weanling rats, supplemental iron form can generate differential effects on the gut microbiota and microbial metabolism that persist into adulthood.

Experiment 1


Needs review

Curated date: 2024/03/16

Curator: Keamy

Revision editor(s): Keamy, Joan Chuks, Victoria

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Rattus norvegicus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Cecum mucosa Caecum mucosa,Caecum mucosa of organ,Caecum mucous membrane,Caecum organ mucosa,Cecal mucosa,Cecum mucosa of organ,Cecum mucous membrane,Cecum organ mucosa,Intestinum crassum caecum mucosa,Intestinum crassum caecum mucosa of organ,Intestinum crassum caecum mucous membrane,Intestinum crassum caecum organ mucosa,Mucosa of caecum,Mucosa of cecum,Mucosa of intestinum crassum caecum,Mucosa of organ of caecum,Mucosa of organ of cecum,Mucosa of organ of intestinum crassum caecum,Mucous membrane of caecum,Mucous membrane of cecum,Mucous membrane of intestinum crassum caecum,Organ mucosa of caecum,Organ mucosa of cecum,Organ mucosa of intestinum crassum caecum,Cecum mucosa,cecum mucosa
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Iron biomarker measurement iron biomarker level,iron status biomarkers,Iron biomarker measurement,iron biomarker measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Vehicle control-supplemented (CON) pups
Group 1 name Corresponds to the case (exposed) group for case-control studies
Ferrous sulfate (FS) treated pups (PD 15)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Pre-weaning Sprague-Dawley rat pups given oral supplements of ferrous sulfate (FS) on postnatal day (PD) 2–14 and samples collected from 4–6 hours fasted animals on postnatal day (PD) 15.
Group 0 sample size Number of subjects in the control (unexposed) group
28
Group 1 sample size Number of subjects in the case (exposed) group
27
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
None

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Richness Number of species
decreased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
decreased

Signature 1

Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Joan Chuks, MyleeeA

Source: Figure 2d + Figure 3 + Table S3 + Table S4

Description: Taxa Differing in Abundance Between Ferrous sulfate (FS) treated pups (PD 15) and Vehicle control-supplemented (CON) pups as determined by DESeq2

Abundance in Group 1: increased abundance in Ferrous sulfate (FS) treated pups (PD 15)

NCBI Quality ControlLinks
Acetatifactor
Aerococcus
Anaeroplasma
Butyricicoccus
Dorea
Faecalibacterium sp. UBA1819
Faecalibaculum
Flavonifractor
Harryflintia
Mycoplasmatota
Papillibacter
Ruminiclostridium
Ruminococcus
Tyzzerella
uncultured Clostridia bacterium
Ruminococcaceae UCG-009Ruminococcaceae UCG-009
Defluviitaleaceae UCG−011Defluviitaleaceae UCG−011
Lachnospiraceae UCG−004Lachnospiraceae UCG−004
Tyzzerella 4Tyzzerella 4
uncultureduncultured
Clostridium

Revision editor(s): Keamy, Joan Chuks, MyleeeA

Signature 2

Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Joan Chuks

Source: Figure 2d + Figure 3 + Table S3 + Table S4

Description: Taxa Differing in Abundance Between Ferrous sulfate (FS) treated pups (PD 15) and Vehicle control-supplemented (CON) pups as determined by DESeq2

Abundance in Group 1: decreased abundance in Ferrous sulfate (FS) treated pups (PD 15)

NCBI Quality ControlLinks
Adlercreutzia
Alistipes
Bifidobacterium
Brachybacterium
Brevibacterium
Butyrivibrio
Candidatus Soleaferrea
Enterococcus
Facklamia
Jeotgalicoccus
Lactobacillus
Marvinbryantia
Massiliimalia
Parasutterella
Staphylococcus
Streptococcus
Turicibacter
Yaniella
uncultured bacterium
uncultured organism
Corynebacterium 1Corynebacterium 1
A2A2
Prevotellaceae NK3B31 groupPrevotellaceae NK3B31 group
Lachnospiraceae UCG−001Lachnospiraceae UCG−001
Coriobacteriaceae UCG−002Coriobacteriaceae UCG−002
Prevotellaceae UCG−001Prevotellaceae UCG−001
Ruminococcus 1Ruminococcus 1
Ruminiclostridium 5Ruminiclostridium 5
Christensenellaceae R−7 groupChristensenellaceae R−7 group

Revision editor(s): Keamy, Joan Chuks

Experiment 2


Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Glorious, Victoria

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
Ferrous bis-glycinate chelate (FC) treated pups (PD 15)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Pre-weaning Sprague-Dawley rat pups given oral supplements of ferrous bis-glycinate chelate (Ferrochel, FC) on postnatal day (PD) and samples collected from 4–6 hours fasted animals on postnatal day (PD) 15.
Group 1 sample size Number of subjects in the case (exposed) group
29

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Richness Number of species
decreased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
decreased

Signature 1

Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Glorious

Source: Figure 2d + Figure 3 + Table S3 + Table S4

Description: Taxa Differing in Abundance Between ferrous bis-glycinate chelate (FC) treated pups (PD 15) and Vehicle control-supplemented (CON) pups as determined by DESeq2

Abundance in Group 1: increased abundance in Ferrous bis-glycinate chelate (FC) treated pups (PD 15)

NCBI Quality ControlLinks
Akkermansia
Allobaculum
Dubosiella
Papillibacter
Ruminococcus gauvreauii
Tyzzerella
Verrucomicrobiota
Ruminococcus 2Ruminococcus 2
Defluviitaleaceae UCG−011Defluviitaleaceae UCG−011
Christensenellaceae R−7 groupChristensenellaceae R−7 group

Revision editor(s): Keamy, Glorious

Signature 2

Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Glorious

Source: Figure 2d + Figure 3 + Table S3 + Table S4

Description: Taxa Differing in Abundance Between ferrous bis-glycinate chelate (FC) treated pups (PD 15) and Vehicle control-supplemented (CON) pups as determined by DESeq2

Abundance in Group 1: decreased abundance in Ferrous bis-glycinate chelate (FC) treated pups (PD 15)

NCBI Quality ControlLinks
Acetatifactor
Adlercreutzia
Alistipes
Bacillota
Bacteroidota
Bifidobacterium
Blautia
Brachybacterium
Brevibacterium
Butyricicoccus
Butyrivibrio
Dorea
Eisenbergiella
Enterococcus
Eubacterium xylanophilum
Facklamia
Jeotgalicoccus
Lachnoclostridium
Lachnospiraceae bacterium NK4A136
Lactobacillus
Marvinbryantia
Massiliimalia
Parabacteroides
Parasutterella
Roseburia
Staphylococcus
Streptococcus
Turicibacter
Yaniella
uncultured Bacteroidales bacterium
uncultured Barnesiella sp.
uncultured bacterium
uncultured organism
unidentified
uncultureduncultured
Family XIII AD3011 groupFamily XIII AD3011 group
Coriobacteriaceae UCG−002Coriobacteriaceae UCG−002
Lachnospiraceae UCG−004Lachnospiraceae UCG−004
Ruminococcus 1Ruminococcus 1
Lachnospiraceae UCG−006Lachnospiraceae UCG−006
Tyzzerella 4Tyzzerella 4
Prevotellaceae NK3B31 groupPrevotellaceae NK3B31 group
Lachnospiraceae UCG−001Lachnospiraceae UCG−001
Lachnospiraceae FCS020 groupLachnospiraceae FCS020 group
Ruminococcaceae NK4A214 groupRuminococcaceae NK4A214 group
Ruminiclostridium 5Ruminiclostridium 5
Prevotellaceae UCG−001Prevotellaceae UCG−001
A2A2
Ruminococcaceae UCG−004Ruminococcaceae UCG−004
Corynebacterium 1Corynebacterium 1
Ruminiclostridium 9Ruminiclostridium 9
uncultured Clostridium sp.

Revision editor(s): Keamy, Glorious

Experiment 3


Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Joan Chuks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Ferrous sulfate (FS) treated pups (PD 15)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Pre-weaning Sprague-Dawley rat pups given oral supplements of ferrous bis-glycinate chelate (Ferrochel, FC) on postnatal day (PD) 2-14 and samples collected from 4–6 hours-fasted animals on postnatal day (PD) 15
Group 0 sample size Number of subjects in the control (unexposed) group
27

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Richness Number of species
decreased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
decreased

Signature 1

Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Joan Chuks

Source: Figure S3 + Table S3 + Table S4

Description: Taxa Differing in Abundance Between Ferrous sulfate (FS) treated pups (PD 15) and Ferrous bis-glycinate chelate (FC) treated pups (PD 15) as determined by DESeq2

Abundance in Group 1: increased abundance in Ferrous bis-glycinate chelate (FC) treated pups (PD 15)

NCBI Quality ControlLinks
Allobaculum
Candidatus Soleaferrea
Dubosiella
Streptococcus
Christensenellaceae R−7 groupChristensenellaceae R−7 group

Revision editor(s): Keamy, Joan Chuks

Signature 2

Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Joan Chuks, MyleeeA

Source: Figure S3 + Table S3 + Table S4

Description: Taxa Differing in Abundance Between Ferrous sulfate (FS) treated pups (PD 15) and Ferrous bis-glycinate chelate (FC) treated pups (PD 15) as determined by DESeq2

Abundance in Group 1: decreased abundance in Ferrous bis-glycinate chelate (FC) treated pups (PD 15)

NCBI Quality ControlLinks
Acetatifactor
Aerococcus
Alistipes
Bacteroides
Bacteroidota
Bifidobacterium
Blautia
Butyricicoccus
Dorea
Eisenbergiella
Eubacterium xylanophilum
Faecalibacterium sp. UBA1819
Haemophilus
Harryflintia
Lachnoclostridium
Lachnospiraceae bacterium NK4A136
Mycoplasmatota
Parabacteroides
Roseburia
uncultured Bacteroidales bacterium
uncultured Barnesiella sp.
uncultured Clostridia bacterium
uncultured Clostridium sp.
uncultured bacterium
uncultured organism
unidentified
Ruminococcus 2Ruminococcus 2
Ruminiclostridium 9Ruminiclostridium 9
Ruminococcaceae UCG−004Ruminococcaceae UCG−004
uncultureduncultured
Ruminococcaceae NK4A214 groupRuminococcaceae NK4A214 group
Lachnospiraceae FCS020 groupLachnospiraceae FCS020 group
Tyzzerella 4Tyzzerella 4
Lachnospiraceae UCG−006Lachnospiraceae UCG−006
Lachnospiraceae UCG−004Lachnospiraceae UCG−004
Prevotellaceae UCG−001Prevotellaceae UCG−001
Ruminiclostridium 6Ruminiclostridium 6
Ruminiclostridium 5Ruminiclostridium 5
Family XIII AD3011 groupFamily XIII AD3011 group
Ruminococcaceae UCG−009Ruminococcaceae UCG−009
Clostridium

Revision editor(s): Keamy, Joan Chuks, MyleeeA

Experiment 4


Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Glorious, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Vehicle control-supplemented (CON) young Adult (YA) rats
Group 1 name Corresponds to the case (exposed) group for case-control studies
Ferrous sulfate (FS) treated young Adult (YA) rats (PD 58 +/- 4)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Young Adult (YA) Sprague-Dawley rats given oral supplements of ferrous sulfate (FS) on postnatal day (PD) 2-20, weaned on postnatal day (PD) 21 and samples collected from 4–6 hours fasted animals on postnatal day (PD) 58 +/- 4.
Group 0 sample size Number of subjects in the control (unexposed) group
23
Group 1 sample size Number of subjects in the case (exposed) group
23

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Richness Number of species
increased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
increased

Signature 1

Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Glorious

Source: Figure 5d and 6 +Figure S6 + Table S7 and S8

Description: Taxa Differing in Abundance Between Young Adult (YA) Sprague-Dawley rats given oral supplements of ferrous sulfate (FS) and Vehicle control-supplemented (CON) as determined by DESeq2

Abundance in Group 1: increased abundance in Ferrous sulfate (FS) treated young Adult (YA) rats (PD 58 +/- 4)

NCBI Quality ControlLinks
Acetatifactor
Enterorhabdus
Allofournierella
Parabacteroides
Rothia
uncultured Firmicutes bacterium
uncultured bacterium
uncultureduncultured
Lachnospiraceae UCG−004Lachnospiraceae UCG−004
Lachnospiraceae NK4B4 groupLachnospiraceae NK4B4 group
Ruminococcaceae NK4A214 groupRuminococcaceae NK4A214 group
Prevotellaceae NK3B31 groupPrevotellaceae NK3B31 group
Ruminococcaceae UCG−009Ruminococcaceae UCG−009
Ruminiclostridium 9Ruminiclostridium 9
uncultured Clostridium spuncultured Clostridium sp
unidentified

Revision editor(s): Keamy, Glorious

Signature 2

Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Glorious

Source: Figure 5d,6 and S6 + Table S7and S8

Description: Taxa Differing in Abundance Between fYoung Adult (YA) Sprague-Dawley rats given oral supplements of ferrous sulfate (FS). and Vehicle control-supplemented (CON)

Abundance in Group 1: decreased abundance in Ferrous sulfate (FS) treated young Adult (YA) rats (PD 58 +/- 4)

NCBI Quality ControlLinks
Acinetobacter
Enterococcus
Escherichia/Shigella sp.
Faecalicatena fissicatena
Lactobacillus
Pseudomonadota
Staphylococcus
Streptococcus
Turicibacter
uncultured bacterium
unidentified
Coprococcus 2Coprococcus 2
Tyzzerella 4Tyzzerella 4
Massiliimalia
Merdimmobilis

Revision editor(s): Keamy, Glorious

Experiment 5


Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Joan Chuks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Vehicle control-supplemented (CON) young Adult rats
Group 1 name Corresponds to the case (exposed) group for case-control studies
Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Young Adult (YA) Sprague-Dawley rats given oral supplements of ferrous bis-glycinate chelate (FC) on postnatal day (PD) 2-20, weaned on postnatal day (PD) 21 and samples collected from 4–6 h-fasted animals on postnatal day (PD) 58 +/- 4.
Group 1 sample size Number of subjects in the case (exposed) group
19

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Richness Number of species
decreased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
decreased

Signature 1

Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Joan Chuks

Source: Figure 5d + Figure 6 + Figure S6 + Table s7 +Table s8

Description: Taxa Differing in Abundance Between Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4) and Vehicle control-supplemented (CON) young Adult rats as determined by DESeq2.

Abundance in Group 1: increased abundance in Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4)

NCBI Quality ControlLinks
Brachybacterium
Brevibacterium
Caldicoprobacter
Cyanobacteriota
Oscillibacter
Papillibacter
Patescibacteria group
Rothia
Ruminiclostridium sufflavum
Tyzzerella
Verrucomicrobiota
Yaniella
uncultured organism
uncultured rumen bacterium
unidentified rumen bacterium RF32
Ruminococcus 1Ruminococcus 1
Defluviitaleaceae UCG−011Defluviitaleaceae UCG−011
uncultureduncultured
Family XIII AD3011 groupFamily XIII AD3011 group
Ruminococcus 2Ruminococcus 2

Revision editor(s): Keamy, Joan Chuks

Signature 2

Needs review

Curated date: 2024/03/20

Curator: Keamy

Revision editor(s): Keamy, Joan Chuks

Source: Figure 5d + Figure 6 + Figure S6 + Table s7 +Table s8

Description: Taxa Differing in Abundance Between Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4) and Vehicle control-supplemented (CON) young Adult rats as determined by DESeq2.

Abundance in Group 1: decreased abundance in Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4)

NCBI Quality ControlLinks
Acetatifactor
Acetitomaculum
Acinetobacter
Adlercreutzia
Alistipes
Anaerostipes
Anaerotruncus
Bacteroides
Bifidobacterium
Blautia
Dorea
Enterorhabdus
Intestinimonas
Lachnoclostridium
Lactobacillus
Parabacteroides
Parasutterella
Roseburia
Turicibacter
uncultured Bacteroidales bacterium
uncultured Barnesiella sp.
uncultured Lachnospiraceae bacterium
Lachnospiraceae UCG−001Lachnospiraceae UCG−001
Ruminiclostridium 9Ruminiclostridium 9
A2A2
Lachnospiraceae FCS020 groupLachnospiraceae FCS020 group
Prevotellaceae UCG−001Prevotellaceae UCG−001
Prevotellaceae NK3B31 groupPrevotellaceae NK3B31 group
Lachnospiraceae UCG−006Lachnospiraceae UCG−006
Ruminococcaceae NK4A214 groupRuminococcaceae NK4A214 group
Coriobacteriaceae UCG−002Coriobacteriaceae UCG−002
Tyzzerella 4Tyzzerella 4
Tyzzerella 3Tyzzerella 3
Lachnospiraceae NK4B4 groupLachnospiraceae NK4B4 group
uncultureduncultured
Lachnospiraceae UCG−004Lachnospiraceae UCG−004
Ruminococcaceae UCG−009Ruminococcaceae UCG−009
Coprococcus 2Coprococcus 2
Actinomycetota
Bacteroidota
Pseudomonadota
Mycoplasmatota
uncultured bacterium
Butyricicoccus
Eubacterium ruminantium
Eubacterium ventriosum
Eubacterium xylanophilum
uncultured Clostridium sp.
Escherichia/Shigella sp.
Massiliimalia
unidentified

Revision editor(s): Keamy, Joan Chuks

Experiment 6


Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Glorious, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Ferrous sulfate (FS) treated young Adult (YA) rats (PD 58 +/- 4)

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Richness Number of species
unchanged
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
unchanged

Signature 1

Needs review

Curated date: 2024/03/19

Curator: Keamy

Revision editor(s): Keamy, Glorious

Source: Figure 5d + Figure 6 + Figure S6 + Table S7 and S8

Description: Taxa Differing in Abundance Between Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4) and Ferrous Sulfate (FS) young Adult rats as determined by DESeq2.

Abundance in Group 1: increased abundance in Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4)

NCBI Quality ControlLinks
Anaerofilum
Anaerofustis
Bacillus
Brachybacterium
Brevibacterium
Caldicoprobacter
Candidatus Saccharimonas
Candidatus Soleaferrea
Cyanobacteriota
Deferribacteres
Dietzia
Enterococcus
Eubacterium coprostanoligenes
Facklamia
Faecalibaculum
Faecalicatena fissicatena
Haemophilus
Hydrogenoanaerobacterium
Lachnospiraceae bacterium NC2004
Moryella
Papillibacter
Patescibacteria group
Ruminiclostridium sufflavum
Ruminococcaceae bacterium UCG-005
Ruminococcus gauvreauii
Sellimonas
Staphylococcus
Verrucomicrobiota
Yaniella
[Eubacterium] nodatum
uncultured organism
uncultured rumen bacterium
unidentified rumen bacterium RF32
Corynebacterium 1Corynebacterium 1
uncultureduncultured
Family XIII AD3011 groupFamily XIII AD3011 group
Defluviitaleaceae UCG−011Defluviitaleaceae UCG−011
Clostridium sensu stricto 18Clostridium sensu stricto 18
Lachnoclostridium 5Lachnoclostridium 5
unidentified

Revision editor(s): Keamy, Glorious

Signature 2

Needs review

Curated date: 2024/03/20

Curator: Keamy

Revision editor(s): Keamy, Glorious

Source: Figure 5d + Figure 6 + Figure S6 + Table S7 andS8

Description: Taxa Differing in Abundance Between Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4) and Ferrous Sulfate (FS) young Adult rats as determined by DESeq2.

Abundance in Group 1: decreased abundance in Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4)

NCBI Quality ControlLinks
Acetatifactor
Acetitomaculum
Actinomycetota
Adlercreutzia
Alistipes
Anaerostipes
Bacteroides
Bacteroidota
Bifidobacterium
Blautia
Butyricicoccus
Clostridiales bacterium enrichment culture clone 06-1235251-67
Dorea
Enterorhabdus
Eubacterium ruminantium
Eubacterium ventriosum
Eubacterium xylanophilum
Intestinimonas
Lachnoclostridium
Massiliimalia
Mycoplasmatota
Parabacteroides
Parasutterella
Roseburia
uncultured Bacteroidales bacterium
uncultured Barnesiella sp.
uncultured Firmicutes bacterium
uncultured Lachnospiraceae bacterium
uncultured Mollicutes bacterium
uncultured bacterium
uncultured rumen bacterium
unidentified
Ruminococcaceae UCG−014Ruminococcaceae UCG−014
uncultureduncultured
Ruminiclostridium 5Ruminiclostridium 5
Ruminococcaceae UCG−009Ruminococcaceae UCG−009
Tyzzerella 3Tyzzerella 3
Lachnospiraceae UCG−004Lachnospiraceae UCG−004
Ruminococcaceae UCG−004Ruminococcaceae UCG−004
Coriobacteriaceae UCG−002Coriobacteriaceae UCG−002
Lachnospiraceae NK4B4 groupLachnospiraceae NK4B4 group
Ruminococcaceae NK4A214 groupRuminococcaceae NK4A214 group
Lachnospiraceae UCG−006Lachnospiraceae UCG−006
Prevotellaceae UCG−001Prevotellaceae UCG−001
Prevotellaceae NK3B31 groupPrevotellaceae NK3B31 group
Lachnospiraceae FCS020 groupLachnospiraceae FCS020 group
Lachnospiraceae UCG−001Lachnospiraceae UCG−001
A2A2
Ruminiclostridium 9Ruminiclostridium 9
uncultured Clostridium sp.

Revision editor(s): Keamy, Glorious