Gut Microbiome Alterations following Postnatal Iron Supplementation Depend on Iron Form and Persist into Adulthood/Experiment 5
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Rattus norvegicus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Cecum mucosa Caecum mucosa,Caecum mucosa of organ,Caecum mucous membrane,Caecum organ mucosa,Cecal mucosa,Cecum mucosa of organ,Cecum mucous membrane,Cecum organ mucosa,Intestinum crassum caecum mucosa,Intestinum crassum caecum mucosa of organ,Intestinum crassum caecum mucous membrane,Intestinum crassum caecum organ mucosa,Mucosa of caecum,Mucosa of cecum,Mucosa of intestinum crassum caecum,Mucosa of organ of caecum,Mucosa of organ of cecum,Mucosa of organ of intestinum crassum caecum,Mucous membrane of caecum,Mucous membrane of cecum,Mucous membrane of intestinum crassum caecum,Organ mucosa of caecum,Organ mucosa of cecum,Organ mucosa of intestinum crassum caecum,Cecum mucosa,cecum mucosa
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Iron biomarker measurement iron biomarker level,iron status biomarkers,Iron biomarker measurement,iron biomarker measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Vehicle control-supplemented (CON) young Adult rats
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Young Adult (YA) Sprague-Dawley rats given oral supplements of ferrous bis-glycinate chelate (FC) on postnatal day (PD) 2-20, weaned on postnatal day (PD) 21 and samples collected from 4–6 h-fasted animals on postnatal day (PD) 58 +/- 4.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 23
- Group 1 sample size Number of subjects in the case (exposed) group
- 19
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Richness Number of species
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased
Signature 1
Source: Figure 5d + Figure 6 + Figure S6 + Table s7 +Table s8
Description: Taxa Differing in Abundance Between Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4) and Vehicle control-supplemented (CON) young Adult rats as determined by DESeq2.
Abundance in Group 1: increased abundance in Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4)
Revision editor(s): Keamy, Joan Chuks
Signature 2
Source: Figure 5d + Figure 6 + Figure S6 + Table s7 +Table s8
Description: Taxa Differing in Abundance Between Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4) and Vehicle control-supplemented (CON) young Adult rats as determined by DESeq2.
Abundance in Group 1: decreased abundance in Ferrous bis-glycinate chelate (FC) treated young Adult (YA) rats (PD 58 +/- 4)
Revision editor(s): Keamy, Joan Chuks
